CX3CL1 and CX3CR1 in the GL261 murine model of glioma: CX3CR1 deficiency does not impact tumor growth or infiltration of microglia and lymphocytes
CX3CL1 and CX3CR1 in the GL261 murine model of glioma: CX3CR1 deficiency does not impact tumor growth or infiltration of microglia and lymphocytes
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DOI：
10.1016/j.jneuroim.2008.04.016
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发表时间：
2008-07-31
影响因子：
3.3
通讯作者：
Harrison, Jeffrey K.
中科院分区：
文献类型：
--
作者：
Liu, Che;Luo, Defang;Harrison, Jeffrey K.
人类多形性胶质母细胞瘤（GBM）是人类脑肿瘤中最恶性的形式。GBM的特征是显著存在肿瘤浸润的小胶质细胞/巨噬细胞和淋巴细胞。本研究的目的是了解趋化因子系统CX 3CL 1及其受体CX 3CR 1在恶性胶质瘤GL 261小鼠模型中的作用。原位杂交分析证实了CX 3CL 1和CX 3CR 1在GL 261肿瘤中的表达。在CX 3CR 1基因破坏的C57 BL/6小鼠中评价CX 3CR 1缺失对颅内GL 261胶质瘤生长和相关免疫细胞浸润的影响。CX 3CR 1-/-小鼠的肿瘤生长速率略有增加，CX 3CR 1 +/-和-/-小鼠中建立的肿瘤内小胶质细胞和CD 4（+）、CD 8（+）、FoxP 3（+）或Ly 49 G2（+）淋巴细胞数量相似。这些数据表明，CX 3CR 1对胶质瘤形成或小胶质细胞和淋巴细胞迁移到GL 261肿瘤中的影响很小或没有影响。(C)2008 Elsevier B. V.保留所有权利。
Human glioblastoma multiforme (GBM) is the most malignant form of human brain tumors. A characteristic of GBM is the marked presence of tumor infiltrated microglia/macrophages and lymphocytes. The goal of this study was directed toward understanding the role of the chemokine system CX3CL1 and its receptor CX3CR1 in the GL261 murine model of malignant glioma. In situ hybridization analysis identified CX3CL1 and CX3CR1 expression in GL261 tumors. The impact of CX3CR1 deletion on the growth of intracranial GL261 gliomas and associated immune cell infiltration was evaluated in CX3CR1 gene-disrupted C57BL/6 mice. A slight increase in the tumor growth rate in CX3CR1-/- mice was evident with similar numbers of microglia and CD4(+), CD8(+), FoxP3(+), or Ly49G2(+) lymphocytes within tumors established in CX3CR1 +/- and -/- mice. These data indicate that CX3CR1 has little or no effects on either gliomagenesis or the migration of microglia and lymphocytes into GL261 tumors. (C) 2008 Elsevier B.V. All rights reserved.