Retinol differentially regulates male germ cell-associated kinase (mak) messenger ribonucleic acid expression during spermatogenesis.
Retinol differentially regulates male germ cell-associated kinase (mak) messenger ribonucleic acid expression during spermatogenesis.
复制标题
视黄醇在精子发生过程中差异性地调节雄性生殖细胞相关激酶 (mak) 信使核糖核酸的表达。
DOI：
10.1095/biolreprod49.5.951
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发表时间：
1993
影响因子：
3.6
通讯作者：
Kim,KH
中科院分区：
文献类型：
--
作者：
Wang,ZQ;Kim,KH
视黄醇在精子发生的原细胞增殖阶段下调雄性生殖细胞相关激酶（mak）转录本。在维生素a缺乏的生殖细胞中检测到2.6和3.4 kb的mak转录本，但在视黄醇替代维生素a缺乏的大鼠后不久就下降到无法检测到的水平。相反，maki似乎在减数分裂和减数分裂后的精母细胞和圆形精母细胞中发挥作用。3.4 kb的转录本最初出现在粗粒精母细胞中，并在圆形精母细胞中继续增加；2.6 kb转录本水平仅在圆形精子中显著增加。这些转录本在同步视黄醇再生的大鼠精管中也表现出阶段特异性。原位杂交后的正常实验显示，在生精周期的第IV-VI期，银颗粒主要集中在圆形精子中。这些结果表明，早期生殖细胞中的mamakgene对视黄醇的反应方式与精母细胞或圆形精子中的mamakgene相反。这些发现支持了睾丸中视黄醇信号的两种不同途径的存在，这两种途径依赖于单个生殖细胞的空间定位和发育年龄。
Retinol down-regulates male germ cell-associated kinase (mak) transcripts during the spermatogonial proliferation phase of spermatogenesis.Maktranscripts of 2.6- and 3.4 kb were detected in vitamin A-deficient germ cells, but decreased to undetectable levels shortly after retinol replacement to vitamin A-deficient rats. In contrast,makseems to play a role in spermatocytes and round spermatids during meiotic and post-meiotic events. The 3.4-kb transcript initially appeared in pachytene spermatocytes and continued to increase in round spermatids; the level of the 2.6-kb transcript increased markedly only in round spermatids. These transcripts also exhibited stage-specificity in synchronized retinol-regenerated rat seminiferous tubules. Silver grains were intensely localized mainly in round spermatids in stages IV-VI of the spermatogenic cycle in normal tests after in situ hybridization. These results suggest that themakgene in early germ cells responds to retinol in an opposite manner from themakgene in spermatocytes or in round spermatids. The findings support the existence of two distinct pathways of retinol signaling in the testis that depend on the spatial localization and development ages of the individual germ cells.