Protective effects of sugar cane extracts (SCE) on Eimeria tenella infection in chickens
Protective effects of sugar cane extracts (SCE) on Eimeria tenella infection in chickens
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DOI：
10.1292/jvms.65.865
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发表时间：
2003-08-01
影响因子：
1.2
通讯作者：
Hirota, Y
中科院分区：
文献类型：
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作者：
El-Abasy, M;Motobu, M;Hirota, Y
通过两个不同的实验研究了口服甘蔗提取物（SCE）对鸡艾美耳球虫卵囊感染的影响。在实验1中，将3周龄的近交鸡（MHC；H.B15）接种到嗉囊中SCE（500 mg/kg/天）1天或连续3天，然后用柔嫩球虫孢子卵囊（2 x 10(4)细胞/鸡）进行攻击。在实验2中，1周龄的鸡连续3天口服相同剂量的SCE，然后最初感染柔嫩球虫孢子卵囊（2×10(3)细胞/鸡）。在2周龄和3周龄时，用绵羊红细胞（SRBC）和流产布鲁氏菌（BA）的混合抗原对这些鸡进行静脉内免疫。 4 周龄时，用柔嫩球虫孢子卵囊攻击鸡（1 x 10(5)/鸡）。感染柔嫩球虫卵囊的鸡表现出每日体重增加显着下降、严重出血、粪便中有大量卵囊脱落以及高病变评分。与其他感染组相比，口服 SCE 和初次感染卵囊（2 x 10(3)/鸡）可显着改善每日体重增加、出血、脱落卵囊数量和病变评分。此外，初次感染的 SCE 接种鸡表现出针对 SRCB 和 BA 的抗体反应显着增加，并且 E. tenella 攻击鸡的盲肠扁桃体淋巴细胞中 Bu-1a(+) 和 CD4 细胞的相对比例下降也有所改善。施用 SCE 并最初感染柔嫩球虫卵囊的鸡的盲肠组织显示，与感染的对照鸡相比，裂殖体、配子体和卵囊的数量较少。这些结果表明，SCE 对鸡的柔嫩艾美耳球虫感染具有免疫刺激和保护作用。
The effects of oral administration of sugar cane extracts (SCE) on Eimeria tenella oocysts infection in chickens were studied with 2 different experiments. In Experiment 1, 3-week-old inbred chickens (MHC; H.B15) were inoculated into the crop with SCE (500 mg/kg/day) for 1 day or 3 consecutive days, and then challenged with E. tenella sporulated oocysts (2 x 10(4) cells/chicken). In Experiment 2, 1-week-old chickens were orally administered SCE at the same dose for 3 consecutive days, and then initially infected with E. tenella sporulated oocysts (2 x 10(3) cells/chicken). At 2 and 3 weeks of age, these chickens were immunized intravenously with the mixed antigens of sheep red blood cells (SRBC) and Brucella abortus (BA). At 4 weeks of age, chickens were challenged with E. tenella sporulated oocysts (1 x 10(5)/chicken). Challenged chickens with E. tenella oocysts showed markedly decreased body weight gain/day, severe hemorrhage and great number of shedding oocysts in feces and high lesion scores. Oral administration of SCE and initial infection with oocysts (2 x 10(3)/chicken) resulted in a remarkable improvement in body weight gain/day, hemorrhage, the number of shedding oocysts and lesion score, compare to other infected groups. In addition, SCE-inoculated chickens with the initial infection showed a significant increase in antibody responses against SRCB and BA and also improvement in decreased relative proportions of Bu-1a(+) and CD4 cells in cecal tonsil lymphocytes of E. tenella-challenged chickens. Cecal tissues of chickens administered SCE and initially infected with E. tenella oocysts showed lower numbers of schizonts, gametocytes and oocysts than those of infected control chickens. These results suggest that SCE have immunostimulating and protective effects against E. tenella infection in chickens.