CHARACTERIZATION OF THE EARLY EVENTS OF POTATO-TUBER DEVELOPMENT
CHARACTERIZATION OF THE EARLY EVENTS OF POTATO-TUBER DEVELOPMENT
复制标题
DOI：
10.1034/j.1399-3054.1991.830405.x
复制
发表时间：
1991-12-01
影响因子：
6.4
通讯作者：
HANNAPEL, DJ
中科院分区：
文献类型：
--
作者：
HANNAPEL, DJ
马铃薯（Solanum tuberosum L. cv. Superior）块茎化的早期事件通过使用叶柄叶单节插条腋芽块茎发育的模型系统进行了检查。 监测鲜重和淀粉积累，以建立形态变化的发育框架。 2天后腋芽鲜重和淀粉含量开始增加。 孵化开始后 4 天即可检测到芽形态的明显变化。 在块茎形态开始时观察到总蛋白和总RNA显着增加。 免疫印迹分析表明，主要的块茎蛋白 patatin 最初可以在第 4 天的芽中检测到，并且 22-kDa 蛋白酶抑制剂最初可以在第 8 天时检测到。Northern 印迹分析证实了两种蛋白质类型在 RNA 水平上的这种积累模式。 两种蛋白酶抑制剂 mRNA 的大量积累发生晚于 patatin mRNA 的积累。 这项研究的结果表明，在块茎发育的早期阶段，在主要块茎储存蛋白大量积累之前，蛋白质和 mRNA 均发生大量积累。
The early events of potato (Solanum tuberosum L. cv. Superior) tuberization were examined by using a model system of axillary bud tuber development from petiole-leaf single-node cuttings. Both fresh weight and starch accumulation were monitored to establish a developmental framework for morphological changes. Fresh weight and starch content began to increase in axillary buds after 2 days. Visible changes in bud morphology could be detected 4 days after the start of incubation. Substantial increases in both total protein and total RNA were observed at the onset of tuber morphology. Immunoblot analysis showed that the major tuber protein, patatin, could be initially detected in day 4 buds and that a 22-kDa proteinase inhibitor could be initially detected at day 8. Northern blot analysis corroborated this pattern of accumulation at the RNA level for both protein types. Substantial accumulation of the two proteinase inhibitor mRNAs occurred later than patatin mRNA accumulation. The results of this study showed that there is considerable accumulation of both protein and mRNA occurring during the early stages of tuber development prior to the substantial accumulation of the major tuber storage proteins.