REDUCTION TO HOMOZYGOSITY INVOLVING P53 IN ESOPHAGEAL CANCERS DEMONSTRATED BY THE POLYMERASE CHAIN-REACTION
REDUCTION TO HOMOZYGOSITY INVOLVING P53 IN ESOPHAGEAL CANCERS DEMONSTRATED BY THE POLYMERASE CHAIN-REACTION
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DOI：
10.1073/pnas.88.11.4976
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发表时间：
1991-06-01
影响因子：
11.1
通讯作者：
RESAU, JH
中科院分区：
文献类型：
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作者：
MELTZER, SJ;YIN, J;RESAU, JH
影响染色体17 p的杂合性缺失在多种人类肿瘤中以高频率检测到，包括结肠癌、乳腺癌、肺癌和脑癌。 这些损失的一个假定的目标是p53，一种位于17 p的肿瘤抑制基因。 据我们所知，杂合性丢失尚未报告在任何位点，包括p53，在人类食管癌。 此外，目前检测杂合性缺失的方法依赖于大量高分子量DNA的可用性，使得小活检标本或石蜡包埋组织的研究成为问题。 我们用聚合酶链反应检测了52例原发性食管癌p53基因杂合性缺失。 一个等位基因的丢失被检测到在52%的信息的情况下，更常见于鳞状细胞癌比腺癌。 Southern印迹分析用于确认聚合酶链反应衍生的数据。 在大约一半的肿瘤中等位基因丢失的鉴定支持了p53失活参与食管癌发病机制的假设。
Loss of heterozygosity affecting chromosome 17p has been detected at high frequencies in a variety of human tumors, including cancers of the colon, breast, lung, and brain. One presumed target of these losses is p53, a tumor suppressor gene located on 17p. To our knowledge, loss of heterozygosity has not yet been reported at any locus, including p53, in human esophageal cancer. Moreover, current methods of detecting loss of heterozygosity depend on the availability of large amounts of high molecular weight DNA, making the study of small biopsy specimens or paraffin-embedded tissues problematic. We examined 52 primary human esophageal neoplasms for loss of heterozygosity affecting the p53 gene by using the polymerase chain reaction. Loss of one allele was detected in 52% of informative cases and was more common in squamous carcinomas than in adenocarcinomas. Southern blot analysis was used to confirm polymerase chain reaction-derived data. The identification of allelic loss in approximately half of the tumors analyzed supports the hypothesis that inactivation of p53 is involved in the pathogenesis of esophageal cancer.