Hydrogen influences HDL-associated enzymes and reduces oxidized phospholipids levels in rats fed with a high-fat diet
Hydrogen influences HDL-associated enzymes and reduces oxidized phospholipids levels in rats fed with a high-fat diet
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氢气影响高密度脂蛋白相关酶并降低高脂饮食大鼠的氧化磷脂水平
DOI：
10.1016/j.lfs.2020.118945
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发表时间：
2021-02-15
期刊：
影响因子：
6.1
通讯作者：
Qin, Shucun
中科院分区：
文献类型：
--
作者：
Liu, Boyan;Zhao, Min;Qin, Shucun
目的：氧化磷脂（OxPL）是氧化应激的结果，可能介导多种病理效应。主要方法：将大鼠随机分为3组：正常对照组、模型组和H-2治疗组，分别给予普通饲料、高脂饲料和4%H-2吸入治疗10周。通过液相色谱-质谱法分析肝脏和血浆中的OxPLs。超离心分离高密度脂蛋白（HDL）。通过蛋白质组学分析，揭示HDL蛋白质组成的变化，并通过低密度脂蛋白氧化实验检测HDL的抗氧化能力。此外，HDL相关酶的活性或表达进行了评估。关键发现：吸入4%H-2降低了大鼠体内OxPLs的积累。体外实验表明，不同浓度的H-2对非酶促氧化介导的OxPLs的形成没有抑制作用。H-2吸入改变了高脂饮食大鼠HDL的成分，增强了其抗氧化能力。进一步的实验表明，H-2可显著调节脂蛋白相关磷脂酶A（2）、对氧磷酶-1的活性以及卵磷脂：胆固醇酰基转移酶的表达。意义：我们的研究结果表明，H-2可能通过影响HDL相关酶而降低OxPLs水平，这表明H-2可用于减轻与氧化应激引起的脂质过氧化相关的疾病。
Aims: Oxidized phospholipids (OxPLs) are formed as a result of oxidative stress, which potentially mediate multiple pathological effects. We aimed to evaluate the effects of hydrogen (H-2) on OxPLs in vivo and the underlying mechanism.Main methods: Rats were randomly assigned to three groups: control group fed with a chow diet, model group fed with a high-fat diet, and H-2-treated group fed with a high-fat diet and treated by 4% H-2 inhalation for ten weeks. OxPLs in liver and plasma were analyzed by liquid chromatography-mass spectrometry. High-density lipoprotein (HDL) was separated by ultracentrifugation. A proteomic analysis was performed to reveal the alternation of HDL protein composition and he antioxidant capacity of HDL was tested by low-density lipoprotein oxidation experiment. Furthermore, the activity or expression of HDL-associated enzymes were evaluated.Key findings: Inhalation of 4% H-2 decreased the accumulation of OxPLs in rats. In vitro tests revealed that the different concentrations of H-2 did not inhibit the formation of OxPLs mediated by non-enzymatic oxidation. H-2 inhalation altered the components and enhanced the anti-oxidative capacity of HDL in rats fed with a high-fat diet. Further experiments showed that H-2 significantly regulated the activity of lipoprotein-associated phospholipase A(2), paraoxonase-1, and the expression of lecithin:cholesterol acyltransferase.Significance: Our findings revealed that H-2 may reduce the OxPLs levels through its influence on HDL-associated enzymes that can act on OxPLs, suggesting that H-2 can be used in alleviating diseases related to lipid peroxidation due to oxidative stress.