Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio
Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio
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DOI：
10.1007/s10126-010-9332-9
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发表时间：
2011-08-01
影响因子：
3
通讯作者：
Sun, Xiao-Wen
中科院分区：
文献类型：
--
作者：
Li, Yan;Xu, Peng;Sun, Xiao-Wen
建立了鲤鱼细菌人工染色体文库。作为正在进行的鲤鱼基因组计划的一部分，该计划旨在组装鲤鱼基因组。该文库包含总共92，160个BAC克隆，平均插入大小为141 kb，构建到BAC载体CopyControl pCC 1BAC上的Hind III限制性位点中，覆盖7.7 X单倍体基因组当量。建立了BAC文库的三维库和超级库，从1/5的BAC文库中筛选出14个靶点的23个阳性克隆。对1,344个克隆的2,688个BAC末端进行了BAC末端测序，获得了2,522个高质量的Q20序列，平均长度为677 bp。测序成功率为93.8%，双末端测序成功率为92.3%。在鲤鱼和斑马鱼的基因组中共发现了212个微共线性，作为对这两个近缘物种进行全基因组比较基因组学研究的尝试。
A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.