Human umbilical cord blood cells alter blood and spleen cell populations after stroke.
Human umbilical cord blood cells alter blood and spleen cell populations after stroke.
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DOI：
10.1007/s12975-012-0208-3
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发表时间：
2012-12
影响因子：
6.9
通讯作者：
Willing, A. E.
中科院分区：
文献类型：
--
作者：
Golden, J. E.;Shahaduzzaman, M.;Wabnitz, A.;Green, S.;Womble, T. A.;Sanberg, P. R.;Pennypacker, K. R.;Willing, A. E.
人脐带血（HUCB）单核细胞（MNC）部分是一种混合细胞群，当静脉注射（i.v）时，可诱导脑卒中啮齿动物模型的功能修复。移植细胞见于梗死半球和脾脏。该项目的目的是确定hub MNCs细胞与脾免疫细胞之间相互作用的性质。雄性Sprague Dawley大鼠接受永久性大脑中动脉闭塞（MCAO）治疗，并在中风后48小时静脉注射两种药物（仅MCAO）、HUCB跨国公司或CD14+单核细胞、CD133+干细胞或CD19+ B细胞缺失的跨国公司。mcao后72小时安乐死，取脾和血MNCs进行流式细胞术和丝裂原增殖测定。所有hub细胞制剂均降低了脾脏T细胞和血液中单核细胞的百分比（p < 0.05）。CD14+和CD19+缺失的MNCs使脾脏巨噬细胞百分比降低（p < 0.001）， CD133缺失的MNCs使脾脏巨噬细胞百分比升高（p < 0.001）；与MCAO后观察到的水平相比，MNC没有改变巨噬细胞的数量。只有HUCB MNC能显著降低ConA诱导的T细胞刺激（p < 0.05）。这些结果表明，hub MNC在脾脏中的作用不是由于单个hub群体，而是所有亚群体的相互作用。
The human umbilical cord blood (HUCB) mononuclear cell (MNC) fraction is a mixed population of cells that induces functional repair in rodent models of stroke when injected intravenously (i.v.). The transplanted cells are found in the infarcted hemisphere and the spleen. The goal of this project was to determine the nature of the interaction between the HUCB MNCs cells and splenic immune cells. Male Sprague Dawley rats underwent permanent middle cerebral artery occlusion (MCAO) and received i.v. injection of either vehicle (MCAO only), HUCB MNCs or MNCs depleted of CD14+ monocytes, CD133+ stem cells or CD19+ B cells 48 hours post-stroke. At 72 hours post-MCAO, the animals were euthanized and the spleens and blood MNCs harvested for flow cytometry and mitogen proliferation assays. All HUCB cell preparations decreased the percentage of T cells in the spleen and monocytes in the blood (p < 0.05). MNCs depleted of CD14+ and CD19+ decreased the percentage of macrophage (p < 0.001), while CD133 depleted MNCs increased the percentage of macrophage in spleen (p < 0.001); MNC did not alter the macrophage population from the level observed after MCAO. Only HUCB MNC significantly decreased Concanavalin A (ConA)-induced T cell stimulation (p < 0.05). These results suggest that the effects of HUCB MNC in the spleen are not due to a single HUCB population, but the interaction of all the subpopulations together.
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影响因子：
14.5
作者：
Lee, Soon-Tae;Chu, Kon;Roh, Jae-Kyu
通讯作者：
Roh, Jae-Kyu
影响因子：
8.3
作者：
Borlongan, CV;Hadman, M;Sanberg, PR
通讯作者：
Sanberg, PR
影响因子：
3.3
作者：
Boltze, Johannes;Schmidt, Uwe R.;Schaebitz, Wolf-Ruediger
通讯作者：
Schaebitz, Wolf-Ruediger
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
15.9
作者：
Taguchi, A;Soma, T;Matsuyama, T
通讯作者：
Matsuyama, T