Hairy root transgene expression analysis of a secretory peroxidase (PvPOX1) from common bean infected by Fusarium wilt
Hairy root transgene expression analysis of a secretory peroxidase (PvPOX1) from common bean infected by Fusarium wilt
复制标题
枯萎病感染的普通豆分泌型过氧化物酶 (PvPOX1) 的毛根转基因表达分析
DOI：
10.1016/j.plantsci.2017.03.011
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发表时间：
2017
期刊：
影响因子：
5.2
通讯作者：
Blair Matthew W.
中科院分区：
文献类型：
--
作者：
Xue Renfeng;Wu Xingbo;Wang Yingjie;Zhuang Yan;Chen Jian;Wu Jing;Ge Weide;Wang Lanfen;Wang Shumin;Blair Matthew W.
植物过氧化物酶（POXs）是植物防御反应中最重要的氧化还原酶之一。然而，大量不同的植物POX基因使得有必要仔细确认每个旁系同源POX基因在特定组织和疾病相互作用中的功能。枯萎病是由尖孢镰刀菌引起的菜豆毁灭性疾病。sp.phaseoli.本研究利用发根农杆菌（Agrobacterium rhizogenes）毛状根转化法，将菜豆抗性基因CAAS 260205的过氧化物酶基因PvPOX 1转化到菜豆感病基因BRB 130中，为PvPOX 1在菜豆抗病性中的作用提供了直接证据。PvPOX1基因在毛状根中的过表达分析表明，PvPOX1基因的过表达提高了转基因植株根部和其余部分对枯萎病的抗性。同时，PvPOX1的表达水平、过氧化物酶活性和过氧化氢（H2O2）的积累也在互作过程中得到增强。结果表明，PvPOX1基因通过活性氧诱导的过敏反应在枯萎病抗性中发挥重要作用。因此，PvPOX1的表达是一个有价值的基因，可用于进一步分析其通过活性氧激活的抗性机制来增强寄主对枯萎病的防御反应。
Plant peroxidases (POXs) are one of the most important redox enzymes in the defense responses. However, the large number of different plant POX genes makes it necessary to carefully confirm the function of each paralogous POX gene in specific tissues and disease interactions. Fusarium wilt is a devastating disease of common bean caused byFusarium oxysporumf. sp.phaseoli. In this study, we evaluated a peroxidase gene,PvPOX1, from a resistant common bean genotype, CAAS260205 and provided direct evidence forPvPOX1’s role in resistance by transforming the resistant allele into a susceptible common bean genotype, BRB130, via hairy root transformation usingAgrobacterium rhizogenes. Analysis ofPvPOX1gene over-expressing hairy roots showed it increased resistance to Fusarium wilt both in the roots and the rest of transgenic plants. Meanwhile, thePvPOX1expressive level, the peroxidase activity and hydrogen peroxide (H2O2) accumulation were also enhanced in the interaction. The result showed that thePvPOX1gene played an essential role in Fusarium wilt resistance through the occurrence of reactive oxygen species (ROS) induced hypersensitive response. Therefore,PvPOX1expression was proven to be a valuable gene for further analysis which can strengthen host defense response against Fusarium wilt through a ROS activated resistance mechanism.