A Portable Fluorescence Camera for Testing Surgical Specimens in the Operating Room: Description and Early Evaluation
A Portable Fluorescence Camera for Testing Surgical Specimens in the Operating Room: Description and Early Evaluation
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DOI：
10.1007/s11307-010-0438-2
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发表时间：
2011-10-01
影响因子：
3.1
通讯作者：
Kobayashi, Hisataka
中科院分区：
文献类型：
--
作者：
Kakareka, John W.;McCann, Thomas E.;Kobayashi, Hisataka
新型光学探针的临床转化需要离体测试人类样本以确保功效。然而，由于监管/隐私问题，可能难以从手术室移除人体组织。因此，我们设计了一台便携式荧光相机，用于在手术室对人体标本进行靶向光学成像探针的测试。使用具有基于罗丹明绿色的可活化成像探针的卵巢癌小鼠异种移植模型来测试该装置。比较市售的成像systems.The原型相机产生的图像与市售的，非便携式imaging systems.We获得的图像相媲美证明了一种基于荧光素的便携式荧光摄像机在手术室中使用的可行性。它的小尺寸确保了从患者身上切除的组织可以在手术室环境中迅速进行荧光测试，从而加快了新型成像探针的测试。
Clinical translation of novel optical probes requires testing of human specimens ex vivo to ensure efficacy. However, it may be difficult to remove human tissue from the operating room due to regulatory/privacy issues. Therefore, we designed a portable fluorescence camera to test targeted optical imaging probes on human specimens in the operating room.A compact benchtop fluorescence camera was designed and built in-house. A mouse xenograft model of ovarian cancer with an activatable imaging probe based on rhodamine green was used to test the device. Comparison was made to commercially available imaging systems.The prototype camera produced images comparable to images acquired with commercially available, non-portable imaging systems.We demonstrate the feasibility of a specimen-based portable fluorescence camera for use in the operating room. Its small size ensures that tissue excised from patients can be tested promptly for fluorescence within the operating room environment, thus expediting the testing of novel imaging probes.