Rab10-Mediated Secretion Synergizes with Tissue Movement to Build a Polarized Basement Membrane Architecture for Organ Morphogenesis.
Rab10-Mediated Secretion Synergizes with Tissue Movement to Build a Polarized Basement Membrane Architecture for Organ Morphogenesis.
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DOI：
10.1016/j.devcel.2016.06.009
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发表时间：
2016-07-11
影响因子：
11.8
通讯作者：
Horne-Badovinac S
中科院分区：
文献类型：
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作者：
Isabella AJ;Horne-Badovinac S
基底膜（BM）是支持上皮功能的平面蛋白质网络。BM结构的调节变化也可以促进组织形态发生，但上皮细胞如何动态重塑其BM尚不清楚。在果蝇中，最初球形卵室的伸长与其周围BM中的纤维极化网络的产生相关。在这里，我们使用实时成像和遗传操作来确定这些原纤维是如何形成的。BM原纤维由卵室上皮细胞之间的细胞周空间中新合成的蛋白质组装而成，并通过定向上皮迁移定向插入BM中。我们发现，Rab 10为基础的分泌途径促进细胞周围BM蛋白的积累和原纤维的形成。最后，通过操纵这一途径，我们表明，BM纤维结构影响卵室形态发生。这项工作突出了调节蛋白质分泌如何与组织运动协同作用，以建立控制组织形状的极化BM架构。Isabella和Horne-Badovinac研究了在果蝇卵室发育的背景下，基底膜（BM）结构如何有助于形态发生。他们描述了滤泡上皮如何协调基于Rab 10的BM蛋白分泌并指导组织迁移以建立极化BM结构。选择性改变这种BM结构会影响器官形态发生。
Basement membranes (BMs) are planar protein networks that support epithelial function. Regulated changes to BM architecture can also contribute to tissue morphogenesis, but how epithelia dynamically remodel their BMs is unknown. In Drosophila, elongation of the initially spherical egg chamber correlates with the generation of a polarized network of fibrils in its surrounding BM. Here, we use live imaging and genetic manipulations to determine how these fibrils form. BM fibrils are assembled from newly synthesized proteins in the pericellular spaces between the egg chamber’s epithelial cells, and undergo oriented insertion into the BM by directed epithelial migration. We find that a Rab10-based secretion pathway promotes pericellular BM protein accumulation and fibril formation. Finally, by manipulating this pathway, we show that BM fibrillar structure influences egg chamber morphogenesis. This work highlights how regulated protein secretion can synergize with tissue movement to build a polarized BM architecture that controls tissue shape. Isabella and Horne-Badovinac examine how basement membrane (BM) structure contributes to morphogenesis in the context of Drosophila egg chamber development. They describe how the follicular epithelium coordinates Rab10-based BM protein secretion and directed tissue migration to build a polarized BM structure. Selectively altering this BM structure affects organ morphogenesis.