Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats
Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats
复制标题
DOI：
10.1016/j.yjmcc.2009.08.004
复制
发表时间：
2009-11-01
影响因子：
5
通讯作者：
Fukuda, Noboru
中科院分区：
文献类型：
--
作者：
Jumabay, Medet;Matsumoto, Taro;Fukuda, Noboru
脂肪组织来源的干细胞已被证明可分化为心肌细胞和血管内皮细胞。在此，我们研究成熟脂肪细胞来源的去分化脂肪(DFAT)细胞能否在体外和体内通过成熟脂肪细胞的上限培养建立DFAT细胞系而分化为心肌细胞。通过对GFP转基因大鼠成熟脂肪细胞的脱分化获得DFAT细胞，通过免疫细胞化学和RT-PCR检测心肌细胞表型标志物，评价DFAT细胞向心肌细胞分化的能力，并观察DFAT细胞移植到大鼠心肌梗死后对心肌细胞再生和血管生成的影响。DFAT细胞在与心肌细胞共培养以及在无心肌细胞的MethoCult培养液中培养时均表达心脏表型标志物，提示DFAT细胞具有向心肌细胞分化的潜能。在大鼠急性心肌梗死模型中，移植的DFAT细胞在梗死心肌中有效积聚，并在细胞移植后8周表达心肌肌节肌动蛋白。
Adipose tissue-derived stem cells have been demonstrated to differentiate into cardiomyocytes and vascular endothelial cells. Here we investigate whether mature adipocyte-derived dedifferentiated fat (DFAT) cells can differentiate to cardiomyocytes in vitro and in vivo by establishing DFAT cell lines via ceiling culture of mature adipocytes. DFAT cells were obtained by dedifferentiation of mature adipocytes from GFP-transgenic rats We evaluated the differentiating ability of DFAT cells into cardiomyocytes by detection of the cardiac phenotype markers in immunocytochemical and RT-PCR analyses in vitro We also examined effects of the transplantation of DFAT cells into the infarcted heart of rats on cardiomyocytes regeneration and angiogenesis. DFAT cells expressed cardiac phenotype markers when cocultured with cardiomyocytes and also when grown in MethoCult medium in the absence of cardiomyocytes, indicating that DFAT cells have the potential to differentiate to cardiomyocyte lineage. In a rat acute myocardial infarction model, transplanted DFAT cells were efficiently accumulated in infarcted myocardium and expressed cardiac sarcomeric actin at 8 weeks after the cell transplantation The transplantation of DFAT cells significantly (p