CXCR4 expression is associated with lymph-node metastasis of oral squamous cell carcinoma.
CXCR4 expression is associated with lymph-node metastasis of oral squamous cell carcinoma.
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DOI：
10.3892/ijo.28.1.61
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发表时间：
2006
影响因子：
5.2
通讯作者：
T. Ishikawa;K. Nakashiro;S. Hara;S. Kłosek;Chunnan Li;S. Shintani;H. Hamakawa
中科院分区：
文献类型：
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作者：
T. Ishikawa;K. Nakashiro;S. Hara;S. Kłosek;Chunnan Li;S. Shintani;H. Hamakawa
口腔鳞状细胞癌淋巴结转移的支持机制尚不清楚。最近的研究表明，在人类的许多恶性肿瘤中，肿瘤细胞表达趋化因子受体并利用趋化因子转移到靶器官。本研究旨在探讨趋化因子及其受体在口腔鳞状细胞癌中的表达和功能。在8个OSCC细胞系中评估趋化因子受体的表达。CXCR 3 mRNA和蛋白在所有检测的OSCC细胞系中表达，而CXCR 4 mRNA和蛋白仅在HSC 2、HSC 3和Ca 9 -22细胞中表达。用CXCR 4的配体，基质细胞衍生因子-1（SDF-1）治疗，增强了表达CXCR 4的OSCC细胞的运动性和侵袭性。然而，CXCR 3配体Mig不影响CXCR 3阳性细胞的迁移或侵袭。我们还评估了CXCR 4表达的临床意义。CXCR 4在90例口腔鳞癌组织中有27例（30%）表达，定位于癌细胞的胞膜和胞浆。CXCR 4表达与淋巴结转移密切相关（P=0.0035）。这些结果提示CXCR 4可能参与了口腔鳞癌的淋巴结转移。
The support mechanisms that are involved in lymph-node metastasis of oral squamous cell carcinoma (OSCC) remain largely unknown. Recent studies have demonstrated that tumor cells express chemokine receptors and use chemokines to metastasize to the target organ in many malignancies in humans. In this study, we examined the expression and function of chemokines and their receptors in OSCC. The expression of chemokine receptors was assessed in eight OSCC cell lines. CXCR3 mRNA and protein were expressed in all the OSCC cell lines examined, while CXCR4 mRNA and protein were expressed only in HSC2, HSC3, and Ca9-22 cells. Treatment with the ligand for CXCR4, stromal cell-derived factor-1 (SDF-1), enhanced the motility and invasiveness of OSCC cells expressing CXCR4. However, the CXCR3 ligand, Mig, did not affect the migration or invasiveness of CXCR3-positive cells. We also evaluated the clinical significance of CXCR4 expression immunohistochemically. CXCR4 expression was detected in 27 (30%) of the 90 OSCC tissues tested, and was localized in the membrane and cytoplasm of cancer cells. There was a highly significant correlation between CXCR4 expression and lymph-node metastasis (P=0.0035). Collectively, these findings suggest that CXCR4 might be involved in the lymph-node metastasis of OSCC.