Select microRNAs are essential for early development in the sea urchin.
Select microRNAs are essential for early development in the sea urchin.
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DOI：
10.1016/j.ydbio.2011.11.015
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发表时间：
2012-02-01
影响因子：
2.7
通讯作者：
Wessel GM
中科院分区：
文献类型：
--
作者：
Song JL;Stoeckius M;Maaskola J;Friedländer M;Stepicheva N;Juliano C;Lebedeva S;Thompson W;Rajewsky N;Wessel GM
microRNA（miRNAs）是一类介导转录后基因调控的非编码小RNA，是许多发育事件的重要调控因子。在早期胚胎发育过程中的紫海胆，球海胆，转录网络，以及描述，将作为一个很好的模型来测试功能的贡献，在胚胎发育的miRNA。我们研究了miRNA生物合成途径主要组分的功能表型的丧失。在胚胎中Drosha和Dicer从头合成的抑制导致一致的发育缺陷，原肠胚形成失败和胚胎致死，包括涉及胚层特化的转录因子和信号分子的稳态水平的变化。我们通过深度测序和计算分析，对卵巢和几个早期胚胎阶段的小RNA表达进行了注释和分析。所有miRNA在早期发育中具有动态积累谱，大量推定的piRNA（piwi相互作用RNA）也是如此。由Drosha缺失引起的形态发生缺陷可以用四种miRNA挽救，这允许强miRNA功能测定。综上所述，我们的研究结果表明，转录后基因调控指导的miRNA是早期胚胎发生的功能重要，是一个组成部分的早期胚胎基因调控网络在S。紫色的
microRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene regulation and have emerged as essential regulators of many developmental events. The transcriptional network during early embryogenesis of the purple sea urchin, Strongylocentrotus purpuratus, is well described and would serve as an excellent model to test functional contributions of miRNAs in embryogenesis. We examined the loss of function phenotypes of the major components of the miRNA biogenesis pathway. Inhibition of de novo synthesis of Drosha and Dicer in the embryo led to consistent developmental defects, a failure to gastrulate, and embryonic lethality, including changes in the steady state levels of transcription factors and signaling molecules involved in germ layer specification. We annotated and profiled small RNA expression from the ovary and several early embryonic stages by deep sequencing followed by computational analysis. All miRNAs have dynamic accumulation profiles through early development as do a large population of putative piRNAs (piwi-interacting RNAs). Defects in morphogenesis caused by loss of Drosha can be rescued with four miRNAs which permits a strong miRNA functional assay. Taken together our results indicate that post-transcriptional gene regulation directed by miRNAs is functionally important for early embryogenesis and is an integral part of the early embryonic gene regulatory network in S. purpuratus.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
64.5
作者：
Han, Jinju;Lee, Yoontae;Kim, V. Narry
通讯作者：
Kim, V. Narry
影响因子：
2.5
作者：
Flowers, VL;Courteau, GR;Venuti, JM
通讯作者：
Venuti, JM
影响因子：
2.7
作者：
Davidson, EH;Rast, JP;Bolouri, H
通讯作者：
Bolouri, H
影响因子：
64.5
作者：
Brennecke, Julius;Aravin, Alexei A.;Hannon, Gregory J.
通讯作者：
Hannon, Gregory J.