Adenovirus-mediated stromal cell-derived factor-1 alpha gene transfer improves cardiac structure and function after experimental myocardial infarction through angiogenic and antifibrotic actions.
Adenovirus-mediated stromal cell-derived factor-1 alpha gene transfer improves cardiac structure and function after experimental myocardial infarction through angiogenic and antifibrotic actions.
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腺病毒介导的基质细胞衍生因子 1 α 基因转移通过血管生成和抗纤维化作用改善实验性心肌梗死后的心脏结构和功能
DOI：
10.1007/s11033-009-9642-z
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发表时间：
2010-04
影响因子：
2.8
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Tang J;Wang J;Song H;Huang Y;Yang J;Kong X;Guo L;Zheng F;Zhang L
基质细胞衍生因子1α（SDF-1 α）不仅是一种主要的趋化因子，而且是血管生成的诱导因子。观察SDF-1α对大鼠心肌梗死（MI）后左室重构的影响。结扎大鼠左冠状动脉诱发心肌梗死。0.5对照组和假手术组分别于梗死区或心肌壁内注射120 μl无细胞PBS，并于梗死后即刻注射10 × 1010 pfu/ml AdV-SDF-1或0.5 × 1010 pfu/ml Adv-LacZ。结果发现AdV-SDF-1组LVSP和±dP/dtmax明显高于对照组和Adv-LacZ组，LVEDP明显低于对照组和Adv-LacZ组。AdV-SDF-1组c-Kit+干细胞数量及SDF-1、VEGF、bFGF基因表达明显增加，与梗死面积缩小、左室壁增厚、血管密度和心肌细胞密度增加有关。AdV-SDF-1组梗死区I型、III型胶原mRNA表达及胶原沉积明显减少，与TGF-β1、TIMP-1、TIMP-2表达减少有关。结论：SDF-1α可通过促血管生成和抗纤维化作用改善心肌梗死后心脏结构和功能。本文的在线版本（doi：10.1007/s11033-009-9642-z）包含补充材料，可供授权用户使用。
Stromal cell-derived factor 1α (SDF-1) is not only a major chemotactic factor, but also an inducer of angiogenesis. The effects of SDF-1α on the left ventricular remodeling in a rat myocardial infarction (MI) model were analyzed. Myocardial infarction was induced by ligation of the left coronary artery in rats. 0.5 × 1010 pfu/ml AdV-SDF-1 or 0.5 × 1010 pfu/ml Adv-LacZ were immediately injected into the infarcted myocardium, 120 μl cell-free PBS were injected into the infarcted region or the myocardial wall in control, and sham group, respectively. We found that AdV-SDF-1 group had higher LVSP and ±dP/dtmax, lower LVEDP compared to control or Adv-LacZ group. The number of c-Kit+ stem cells, and gene expression of SDF-1, VEGF and bFGF were obviously increased, which was associated with reduced infarct size, thicker left ventricle wall, greater vascular density and cardiocytes density in infarcted hearts of AdV-SDF-1 group. Furthermore, the expression of collagen type I and type III mRNA, and collagen accumulation in the infarcted area was lower, which was associated with decreased TGF-β1, TIMP-1 and TIMP-2 expression in AdV-SDF-1 group. Conclusion: SDF-1α could improve cardiac structure and function after Myocardial infarction through angiogenic and anti-fibrotic actions. The online version of this article (doi:10.1007/s11033-009-9642-z) contains supplementary material, which is available to authorized users.
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影响因子：
9.5
作者：
Ma, J;Ge, JB;Zou, YZ
通讯作者：
Zou, YZ
影响因子：
2.2
作者：
Bloomston, M;Shafii, S;Rosemurgy, AS
通讯作者：
Rosemurgy, AS
影响因子：
37.8
作者：
Cimini, Massimo;Fazel, Shafie;Li, Ren-Ke
通讯作者：
Li, Ren-Ke
DOI：
10.1152/ajpheart.00370.2004
发表时间：
2005-01-01
影响因子：
4.8
作者：
Ikonomidis, JS;Hendrick, JW;Spinale, FG
通讯作者：
Spinale, FG
影响因子：
9.5
作者：
Koch, KC;Schaefer, WM;Weber, C
通讯作者：
Weber, C