Comparison of serological, culture, and bait methods for detection of Pythium and Phytophthora zoospores in water
Comparison of serological, culture, and bait methods for detection of Pythium and Phytophthora zoospores in water
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DOI：
10.1046/j.1365-3059.2002.00759.x
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发表时间：
2002-12
期刊：
影响因子：
2.7
通讯作者：
T. Pettitt;A. Wakeham;M. Wainwright;J. G. White
中科院分区：
文献类型：
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作者：
T. Pettitt;A. Wakeham;M. Wainwright;J. G. White
比较了两种免疫诊断检测方法与两种常规检测方法在无菌蒸汽水稀释系列中检测孢子囊孢霉、F型霉、疫霉和隐孢霉繁殖体的敏感性。对四种细菌最敏感的方法是游动孢子捕获免疫分析法（ZTI）。传统的膜过滤-稀释镀对两种疫霉菌的检测结果与ZTI相似，但对检出的Pythium的敏感性较低。免疫诊断试纸试验和常规诱饵试验在稀释系列中显示相似的敏感性，并且通常比ZTI低两个数量级。并比较了4种检测方法对园艺苗圃水样的检测效果，以及对樟子树、番茄和菊花病害根区的原位检测效果。在这些比较中，ZTI再次成为水样最敏感的测试，尽管膜过滤-稀释电镀被证明是更一致的测试。量尺法和诱饵法是原位检测的最佳技术，量尺法提供了流行病学上有价值的病原体繁殖体数量的定量数据。
Two immunodiagnostic detection assay procedures were compared with two conventional assays for their sensitivity in detecting propagules of Pythium ultimum var. sporangiiferum, Pythium Group F, Phytophthora cactorum and P. cryptogea in dilution series in sterile distilled water. The most sensitive assay for all four species was the zoospore trapping immunoassay (ZTI). Conventional membrane filtration-dilution plating gave similar results to ZTI with the two Phytophthora spp., but was less sensitive in Pythium detection. Immunodiagnostic dipstick assays and conventional bait tests showed similar sensitivities in the dilution series, and were generally about two orders of magnitude less sensitive than ZTI. The four techniques were also compared for their detection efficacy with water samples collected from horticultural nurseries and in in situ tests of infected root zones of Chamaecyparis, tomato and Chrysanthemum. In these comparisons, ZTI was again the most sensitive test for water samples, although membrane filtration-dilution plating proved to be a more consistent test. Dipstick and baiting assays were the best techniques for in situ testing, and dipsticks provided epidemiologically valuable, quantitative data on pathogen propagule numbers.