The effects of a sudden salinity change on cortisol, glucose, lactate, and osmolality levels in grouper Epinephelus malabaricus
The effects of a sudden salinity change on cortisol, glucose, lactate, and osmolality levels in grouper Epinephelus malabaricus
复制标题
盐度突然变化对石斑鱼皮质醇、葡萄糖、乳酸和渗透压水平的影响
DOI：
10.1007/s10695-012-9620-6
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发表时间：
2012
影响因子：
2.9
通讯作者：
Sha
中科院分区：
文献类型：
--
作者：
Wen;Jiann;Sha
将先前保存在24‰海水中的石斑鱼（重46.37±5.10克）转移到14、19、24（对照）、29和34‰海水中。在 240 分钟内的 7 个时间点测量血清皮质醇、葡萄糖、乳酸和渗透压水平。转移到29和34‰海水中的鱼的血清皮质醇和葡萄糖水平分别在10和20分钟后显着升高至最高。 30 分钟后，所有处理中的鱼的血清皮质醇和葡萄糖水平均未观察到显着差异。转移到14、19、29和34‰海水中10-30分钟后，鱼的血清乳酸水平显着低于对照鱼。然而，在 60 分钟内，五种处理之间的血清乳酸没有观察到显着差异。 240分钟转移后鱼的血清渗透压随盐度直接增加，而240分钟转移后鱼的渗透调节能力值（中等渗透压-血浆渗透压）与盐度成反比。得出结论，石斑鱼在14-34‰的海水中表现出较强的渗透调节能力。转移到29%和34%海水中的鱼的血清皮质醇和葡萄糖水平在10-30分钟内迅速增加，表明早期的应激反应。
GrouperEpinephelus malabaricus(weighing 46.37 ± 5.10 g) previously maintained in 24‰ seawater were transferred to 14, 19, 24 (control), 29, and 34‰ seawater. Serum cortisol, glucose, lactate, and osmolality levels were measured at 7 time points during 240 min. Serum cortisol and glucose levels of fish transferred to 29 and 34‰ seawater significantly increased to the highest after 10 and 20 min, respectively. No significant differences in serum cortisol and glucose levels were observed for the fish after 30 min among all treatments. Serum lactate level of fish transferred to 14, 19, 29, and 34‰ seawater was significantly lower than that of the control fish after 10–30 min. However, no significant differences in serum lactate were observed 60 min among five treatments. The serum osmolality of the fish following 240-min transfer increased directly with salinity, whereas the osmoregulatory capacity value (medium osmolality−plasma osmolality) of the fish following 240-min transfer was inversely related to salinity. It is concluded that grouper showed strong osmoregulation in 14–34‰ seawater. Serum cortisol and glucose levels of fish transferred to 29 and 34‰ seawater increased rapidly in 10–30 min, indicating an early stress response.