Neuropeptide Y overexpression in the preweanling Zucker (fa/fa) rat.
Neuropeptide Y overexpression in the preweanling Zucker (fa/fa) rat.
复制标题
神经肽 Y 在断奶前 Zucker (fa/fa) 大鼠中过度表达。
DOI：
10.1016/s0031-9384(99)00095-5
复制
发表时间：
1999
影响因子：
2.9
通讯作者：
Smith,GP
中科院分区：
文献类型：
--
作者：
Kowalski,TJ;Houpt,TA;Jahng,J;Okada,N;Liu,SM;ChuaJr,SC;Smith,GP
检查Zucker脂肪（fa/fa）大鼠下丘脑前体NPY过表达。原位杂交被用来确定在弓状核的+/+，+/法，和法/法年龄出生后第2天（P2），5，9，12，或25的幼仔preproNPY mRNA的相对水平。测量弓状核中探针杂交的相对光密度（ROD）、杂交面积（A）和ROD × A的乘积（总弓状核前体NPY mRNA杂交的量度）。将数值标准化为每窝内的平均+/fa值。初步分析显示，在P9、12和25时，fa/fa幼仔中的preproNPY mRNA杂交（ROD × A）显著高于+/fa和+/+幼仔，并且在P5时显著高于+/fa。然而，在任何测试的年龄，瘦型（+/+和+/fa）基因型之间没有显着差异。因此，将来自瘦型基因型的值合并，并将数据标准化为瘦型动物的平均值用于分析。该分析显示，早在P2，FA/FA幼仔中的preproNPY mRNA杂交高于瘦同窝仔。
Hypothalamic preproNPY overexpression in the Zucker fatty (fa/fa) rat was examined. In situ hybridization was used to determine the relative level of preproNPY mRNA in the arcuate nucleus of +/+, +/fa, and fa/fa pups aged postnatal day 2 (P2), 5, 9, 12, or 25. The relative optical density (ROD) of probe hybridization in the arcuate, the area of hybridization (A), and the product of ROD × A (a measure of total arcuate preproNPY mRNA hybridization) were measured. Values were normalized to the mean +/fa value within each litter. Initial analysis showed that preproNPY mRNA hybridization (ROD × A) in fa/fa pups was significantly higher than +/fa and +/+ pups on P9, 12, and 25, and significantly higher than +/fa on P5. No significant difference between lean (+/+ and +/fa) genotypes, however, were observed at any age tested. Values from the lean genotypes were, therefore, pooled, and data were normalized to the mean value of lean animals for analysis. This analysis revealed that preproNPY mRNA hybridization in fa/fa pups was higher than lean littermates as early as P2.