A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome
A highly-sensitive leakage current microsensor by using denaturant: detection of target protein by DPPC liposome
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使用变性剂的高灵敏漏电流微传感器：DPPC脂质体检测目标蛋白
DOI：
10.1016/j.proeng.2010.09.232
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. Noda
中科院分区：
文献类型：
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作者：
P. Lorchirachoonkul;T. Shimanouchi;K. Yamashita;H. Umakoshi;R. Kuboi;M. Noda
利用DPPC脂质体包埋K_4 [Fe（CN）_6]溶液，研制了一种高灵敏度的微漏电流传感器，用于检测生物分子尤其是蛋白质的存在及其动态状态。在这项工作中，盐酸胍（GuHCl）作为蛋白变性剂的加入成功地获得了显着的灵敏度提高了129倍的幅度，虽然目标蛋白，这是从牛（CAB）的碳酸酐酶，在正常条件下与脂质体的弱相互作用。此外，我们可以用它来评估CAB蛋白的构象状态，如“天然”，“熔融球”和“展开”状态。
We have developed a highly-sensitive leakage current microsensor by using the DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully obtain a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as ‘Native’, ‘Molten-Globule’ and ‘Unfold’ states.
通过检测电解质捕获脂质体与蛋白质之间相互作用的漏电流来构建蛋白质微传感器"
DOI：
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发表时间：
2009
期刊：
影响因子：
--
作者：
M. Noda;T. Asai;T. Shimanouchi;K. Yamashita;H. Umakoshi;M. Okuyama;R. Kuboi
通讯作者：
R. Kuboi