MOBILIZATION OF THE GYPSY-RETROTRANSPONSON AND COPIA-RETROTRANSPOSON IN DROSOPHILA-MELANOGASTER INDUCES REVERSION OF THE OVOD DOMINANT FEMALE-STERILE MUTATIONS - MOLECULAR ANALYSIS OF REVERTANT ALLELES
MOBILIZATION OF THE GYPSY-RETROTRANSPONSON AND COPIA-RETROTRANSPOSON IN DROSOPHILA-MELANOGASTER INDUCES REVERSION OF THE OVOD DOMINANT FEMALE-STERILE MUTATIONS - MOLECULAR ANALYSIS OF REVERTANT ALLELES
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DOI：
10.1002/j.1460-2075.1989.tb03539.x
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发表时间：
1989-05-01
期刊：
影响因子：
11.4
通讯作者：
GANS, M
中科院分区：
文献类型：
--
作者：
MEVELNINIO, M;MARIOL, MC;GANS, M
卵基因座是维持果蝇雌性生殖系所必需的。在缺乏卵+基因的情况下，男性是完全正常的，但女性没有生殖系干细胞。当ovoD雄性与yv f mal菌株的雌性杂交时，观察到卵基因座上的三个显性突变（称为ovoD）以高频率回复到隐性等位基因。我们发现该菌株含有大量的吉普赛转座因子，将其与ovoD菌株杂交会导致吉普赛和copia的移动，并在卵基因座上高频率插入：在检查的16例回复突变中，12例为吉普赛人，4例为4E（卵细胞学位点）插入的copia。使用吉普赛DNA作为标签，我们已经克隆了32 kb的野生型DNA序列周围的吉普赛插入和分子重排的特点在几个独立的回复突变体：在其中10吉普赛似乎被插入到相同的网站。回复突变体中是否出现相邻的菱形突变与吉普赛人的取向密切相关。从影响紧密侧翼区域的缺失的断点分子上确定卵位点的远端限制。
The ovo locus is required for the maintenance of the female germ line in Drosophila melanogaster. In the absence of an ovo+ gene, males are completely normal but females have no germ-line stem cells. Three dominant mutations at the ovo locus, called ovoD, were observed to revert towards recessive alleles at high frequency when ovoD males were crossed to females of the strain y v f mal. We have found that this strain contains an inordinately high number of gypsy transposable elements, and crossing it with the ovoD strains results in the mobilization of both gypsy and copia, with high-frequency insertions into the ovo locus: of 16 revertans examined 12 have gypsy and four have copia inserted at 4E, the ovo cytological site. Using gypsy DNA as a tag we have cloned 32 kb of wild-type DNA sequences surrounding a gypsy insertion and characterized molecular rearrangements in several independent revertants: in 10 of them gypsy appears to be inserted into the same site. The orientation of gypsy is strictly correlated with whether the neighbouring lozenge-like mutation appears in the revertants. A distal limit of the ovo locus was molecularly determined from the breakpoint of a deletion affecting closely flanking regions.