Neutrophil CD11b, L-selectin and Fc IgA receptors in patients with dermatitis herpetiformis.
Neutrophil CD11b, L-selectin and Fc IgA receptors in patients with dermatitis herpetiformis.
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疱疹样皮炎患者的中性粒细胞 CD11b、L-选择素和 Fc IgA 受体。
DOI：
10.1046/j.1365-2133.2002.05004.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Hall3rd,RP
中科院分区：
文献类型：
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作者：
Smith,AD;Streilein,RD;Hall3rd,RP
背景疱疹样皮炎（DH）患者皮损的特征是在诊断性皮肤伊加沉积区域的真皮乳头尖端存在嗜中性粒细胞。尽管已知DH皮损的存在与麸质敏感性肠病相关，控制皮肤病变发展的机制尚不清楚。目的：确定DH患者的循环中性粒细胞是否有启动的证据，如CD 11b表达增加，L-选择素表达减少，中性粒细胞Fc伊加受体功能增加。方法采用荧光激活细胞分选仪检测12例正常受试者和10例活动性、持续性疾病和14例静止性疾病活动性DH患者的中性粒细胞表面CD 11b，L-选择素表达，Fc伊加表达（CD 89）和Fc伊加受体的功能，通过确定中性粒细胞的单克隆人伊加.ResultsNeutrophils的结合能力，进行性DH患者的中性粒细胞与非活动性DH患者或正常受试者相比，CD 11b的表达增加[平均净几何平均通道荧光（GMCF）：活动性DH为403·3，非活动性DH为237·8，正常人为290·5，P< 0·05]。两组DH患者的L-选择素表达均显著低于正常受试者（平均净GMCF：活动性DH，363·2;非活动性DH，375·2;正常受试者，432·7;P< 0·05）。CD 89的表达在任何组中没有差异，但是，Fc伊加受体的功能增加与活动性DH患者相比，与非活动性DH和正常subjects.ConclusionsNeutrophils患者的活动性，进行性DH显示CD 11b的表达增加，L-选择素的表达减少和结合伊加的能力增加，与中性粒细胞的启动模式一致。这种引发可能发生在肠道中，这是由于持续的粘膜免疫应答，这种免疫应答存在于吃含麸质饮食的DH患者中，并且可能使中性粒细胞倾向于定位在DH患者的皮肤中。
SummaryBackgroundThe skin lesions found in patients with dermatitis herpetiformis (DH) are characterized by the presence of neutrophils at the dermal papillary tips in areas where the diagnostic cutaneous IgA deposits are found. Although the presence of the skin lesions of DH is known to be associated with gluten‐sensitive enteropathy, the mechanisms that control the development of skin lesions are not known.ObjectivesTo determine if circulating neutrophils from patients with DH have evidence of priming as shown by increased expression of CD11b, decreased expression of L‐selectin and increased function of neutrophil Fc IgA receptor.MethodsNeutrophils from 12 normal subjects and 10 DH patients with active, ongoing disease and 14 DH patients with quiescent disease activity were examined by fluorescence‐activated cell sorter for expression of cell surface CD11b, L‐selectin expression, Fc IgA expression (CD89) and for the function of the Fc IgA receptor by determining the binding capacity of neutrophils for monoclonal human IgA.ResultsNeutrophils from patients with active, ongoing DH had increased expression of CD11b when compared with patients with inactive DH or normal subjects [mean net geometric mean channel fluorescence (GMCF): active DH, 403·3; inactive DH, 237·8; normal subjects, 290·5;P< 0·05]. L‐selectin expression in both groups of DH patients was significantly lower than that seen in normal subjects (mean net GMCF: active DH, 363·2; inactive DH, 375·2; normal subjects, 432·7;P< 0·05). No difference in CD89 expression was seen in any of the groups; however, the function of Fc IgA receptor was increased in patients with active DH when compared with patients with inactive DH and normal subjects.ConclusionsNeutrophils from patients with active, ongoing DH show an increased expression of CD11b, decreased expression of L‐selectin and increased ability to bind IgA, consistent with a pattern of priming of the neutrophils. This priming may occur in the gut as a result of the ongoing mucosal immune response that is present in patients with DH on a gluten‐containing diet and may predispose neutrophils to localize in the skin of patients with DH.