Coordinate gene expression of luteinizing hormone-releasing hormone (LHRH) and the LHRH-receptor after prolactin stimulation in the rat Nb2 T-cell line: implications for a role in immunomodulation and cell cycle gene expression.
Coordinate gene expression of luteinizing hormone-releasing hormone (LHRH) and the LHRH-receptor after prolactin stimulation in the rat Nb2 T-cell line: implications for a role in immunomodulation and cell cycle gene expression.
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DOI：
10.1210/mend.9.1.7760850
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发表时间：
1995
影响因子：
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通讯作者：
T. Wilson;L. Yu-Lee;M. Kelley
中科院分区：
文献类型：
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作者：
T. Wilson;L. Yu-Lee;M. Kelley
已显示PRL在刺激静止的Nb 2 T细胞后诱导许多基因，包括c-fos、c-myc、鸟氨酸脱羧酶、干扰素调节因子-1等。这些基因之一，LHRH，以前没有报道过以这种方式响应，尽管我们和其他人已经报道了它在大鼠和人类T细胞和B细胞中的存在。此外，最近的证据表明，LHRH作为一种免疫调节剂的功能，在一个类似的方式。利用大鼠未成熟T细胞系Nb 2，我们首次提供的数据表明：1）LHRH基因在细胞周期的不同时间受到PRL的调控; 2）Nb 2细胞中存在选择性剪接的LHRH信使RNA，并可能产生新的截短的GnRH相关肽（也称为PIF的PRL抑制因子）; 3）LHRH受体在加入PRL后以类似于LHRH基因的方式在淋巴细胞中表达，其互补DNA序列与垂体受体的互补DNA序列相同; 5）在LHRH基因的相对链上发现的SH基因与LHRH基因同时并以相同的方式在淋巴细胞中表达; 6）LHRH信使RNA在这些细胞中具有非常短的半衰期; 7）淋巴细胞LHRH转录起始位点与下丘脑位点基本相同。这些数据加强了免疫系统中PRL和LHRH表达之间的关系，并进一步支持我们的论点，即LHRH是一种重要的免疫调节剂，与其他已知的细胞因子相当。
PRL has been shown to induce a number of genes after the stimulation of quiescent Nb2 T-cells, including c-fos, c-myc, ornithine decarboxylase, interferon regulatory factor-1, and others. One of these genes, LHRH, has not previously been reported to respond in this manner, although we and others have reported its presence in rat and human T- and B-cells. Furthermore, recent evidence suggests that LHRH functions as an immunoregulator in a cytokine-like manner. Using the rat immature T-cell line Nb2, we present data showing for the first time that 1) the LHRH gene is regulated by PRL at various times during the cell cycle; 2) an alternatively spliced LHRH messenger RNA exists in Nb2 cells and may produce a new truncated GnRH-associated peptide (alternatively called PIF for PRL-inhibiting factor); 3) the LHRH receptor is expressed in lymphocytes in a manner similar to the LHRH gene after PRL addition, and its complementary DNA sequence is identical to that of the pituitary receptor; 5) the SH gene, found on the opposite strand of the LHRH gene, is expressed in lymphocytes at the same time and in the same manner as the LHRH gene; 6) the LHRH messenger RNA has a very short half-life in these cells; and 7) the lymphocyte LHRH transcription start site is essentially the same as the hypothalamic site. These data strengthen the relationship between PRL and LHRH expression in the immune system and further support our contention that LHRH is an important immunoregulator, on par with other known cytokines.