Effects of chlorhexidine, minocycline, and metronidazole on Porphyromonas gingivalis strain 381 in Biofilms
Effects of chlorhexidine, minocycline, and metronidazole on Porphyromonas gingivalis strain 381 in Biofilms
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DOI：
10.1902/jop.2003.74.11.1647
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发表时间：
2003-11-01
影响因子：
4.3
通讯作者：
Ebisu, S
中科院分区：
文献类型：
--
作者：
Noiri, Y;Okami, Y;Ebisu, S
背景：牙龈卟啉单胞菌存在于龈下生物膜中，与牙周病有关。生物膜中的细菌能够抵抗比悬浮液中更高的抗菌剂浓度。关于生物膜中牙龈卟啉单胞菌对抗菌药物的敏感性知之甚少。方法：将牙龈卟啉单胞菌381株在32个羟基磷灰石片上制备生物膜，观察葡萄糖酸氯己定、盐酸米诺环素和甲硝唑对生物膜的影响。在0，24，72，和144小时后灌注的三种抗菌剂，两个磁盘从每个设备被用来评估的抗菌效果的三磷酸腺苷（ATP）的生物发光，并通过扫描电子显微镜（SEM）的形态调查。显著下降（P
Background: Porphyromonas gingivalls is found in subgingival biofilm and is associated with periodontal disease. Bacteria in biofilms are able to resist higher antimicrobial concentrations than in suspension. Little is known about the susceptibility of P gingivalis in biofilms to antimicrobial agents. The effects of chlorhexidine gluconate, minocycline hydrochloride, and metronidazole on P gingivalis biofilms were examined in vitro.Methods: P gingivalis strain 381 biofilms were prepared on 32 hydroxyapatite disks. At 0, 24, 72, and 144 hours after perfusion of the three antimicrobial agents, two disks from each device were used to assess the antimicrobial effects by adenosine triphosphate (ATP) bioluminescence, and for morphological investigation by scanning electron microscopy (SEM).Results: Close relationships were found between the results of the ATP analyses and the SEM observations in all groups examined. A significant decrease (P