Generation of leukotriene C4 from a subclass of mast cells differentiated in vitro from mouse bone marrow.
Generation of leukotriene C4 from a subclass of mast cells differentiated in vitro from mouse bone marrow.
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从小鼠骨髓体外分化的肥大细胞亚类产生白三烯 C4。
DOI：
10.1073/pnas.79.15.4665
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发表时间：
1982
影响因子：
11.1
通讯作者：
Austen,KF
中科院分区：
文献类型：
--
作者：
Razin,E;Mencia-Huerta,JM;Lewis,RA;Corey,EJ;Austen,KF
骨髓来源的肥大细胞，在体外分化，表现出表面IgE，受体和含有组胺的异染颗粒，这是由硫酸软骨素E蛋白聚糖，而不是肝素蛋白聚糖。用钙离子载体A23187激活这一亚类肥大细胞导致免疫反应性C-6-硫肽白三烯以剂量和时间依赖性方式产生。通过反相高效液相色谱法（RP-HPLC）分离免疫反应性C-6-磺肽白三烯，其保留时间和特异性生物活性与合成的白三烯C4（LTC 4）相同。在上清液的RP-HPLC分离期间，未识别[3 H] LTC 4/LTC 4的放射性标记或免疫反应性转化产物。新鲜骨髓培养物未能产生C-6-硫肽白三烯响应离子载体表明，白三烯的产生依赖于细胞分化成肥大细胞群体。在最佳离子载体刺激过程中产生的LTC 4的量为90.9 +/- 7.5 ng/10（6）个细胞，与常规含肝素大鼠肥大细胞或小鼠肥大细胞瘤细胞产生的C-6-硫代肽白三烯的相对低的量形成对比。
Bone marrow-derived mast cells, differentiated in vitro, demonstrate surface IgE, receptors and contain histamine in metachromatic granules, which are composed of chondroitin sulfate E proteoglycan rather than heparin proteoglycan. Activation of this subclass of mast cells with calcium ionophore A23187 resulted in generation of immunoreactive C-6-sulfidopeptide leukotriene in a dose- and time-dependent fashion. Isolation of immunoreactive C-6-sulfldopeptide leukotriene by reverse-phase high-performance liquid chromatography (RP-HPLC) revealed a retention time and a specific biologic activity identical to those of synthetic leukotriene C4 (LTC4). Neither radiolabeled nor immunoreactive conversion products of [3H]LTC4/LTC4 were recognized during RP-HPLC resolution of the supernatants. The failure of fresh bone marrow cultures to generate C-6-sulfidopeptide leukotriene in response to ionophore indicates that leukotriene generation is dependent upon cellular differentiation into a mast cell population. The amount of LTC4 generated during optimal ionophore stimulation, 90.9 +/- 7.5 ng per 10(6) cells, contrasts with the relatively low amounts of C-6-sulfidopeptide leukotriene generated by the conventional heparin-containing rat mast cells or mouse mastocytoma cells.