Cloning of a novel somatostatin receptor, SSTR3, coupled to adenylylcyclase.
Cloning of a novel somatostatin receptor, SSTR3, coupled to adenylylcyclase.
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发表时间：
1992-10
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通讯作者：
K. Yasuda;S. Rens‐Domiano;C. Breder;S. Law;C. Saper;T. Reisine;G. Bell
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作者：
K. Yasuda;S. Rens‐Domiano;C. Breder;S. Law;C. Saper;T. Reisine;G. Bell
编码新型小鼠生长抑素受体（称为 mSSTR3）的基因已被分离和表征。 mSSTR3 的序列与 mSSTR1 和 mSSTR2 分别显示 46% 和 47% 的同一性。 mSSTR3 以高亲和力结合生长抑素-14 和生长抑素-28，但与生长抑素类似物 MK-678 和 SMS-201-995 的亲和力非常低。此外，mSSTR3 与百日咳毒素敏感的 G 蛋白偶联，并介导生长抑素对毛喉素刺激和多巴胺 D1 受体刺激的 cAMP 形成的抑制，表明它与腺苷酸环化酶偶联。 mSSTR3 的药理学特性及其与腺苷酸环化酶偶联的能力将 SSTR3 与其他克隆的生长抑素受体区分开来，并表明它介导与 SSTR1 或 SSTR2 不同的生物学功能。原位杂交表明SSTR3 mRNA在小鼠大脑中广泛分布，并且其在外侧嗅束核和啮齿动物大脑初级嗅皮层梨状皮层中的表达表明SSTR3可能参与初级感觉信息的处理和调节。
The gene encoding a novel mouse somatostatin receptor termed mSSTR3 was isolated and characterized. The sequence of mSSTR3 shows 46 and 47% identity with mSSTR1 and mSSTR2, respectively. mSSTR3 binds somatostatin-14 and somatostatin-28 with high affinity, but shows very low affinity for the somatostatin analogs MK-678 and SMS-201-995. In addition, mSSTR3 is coupled to pertussis toxin-sensitive G proteins and mediates somatostatin inhibition of forskolin-stimulated and dopamine D1 receptor-stimulated cAMP formation, indicating that it is coupled to adenylylcyclase. The pharmacological properties of mSSTR3 and its ability to couple with adenylylcyclase distinguish SSTR3 from the other cloned somatostatin receptors and indicates that it mediates biological functions different from SSTR1 or SSTR2. In situ hybridization indicates that SSTR3 mRNA is widely distributed in the mouse brain, and its expression in the nucleus of the lateral olfactory tract and in the piriform cortex, the primary olfactory cortex in the rodent brain, suggests that SSTR3 may participate in the processing and modulation of primary sensory information.