Mutagenesis of the cyanobacterium Spirulina platensis by UV and nitrosoguanidine treatment.
Mutagenesis of the cyanobacterium Spirulina platensis by UV and nitrosoguanidine treatment.
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DOI：
10.1111/j.1574-6968.1991.tb04394.x
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发表时间：
1991-09
影响因子：
2.1
通讯作者：
L. Lanfaloni;M. Trinei;M. Russo;C. Gualerzi
中科院分区：
文献类型：
--
作者：
L. Lanfaloni;M. Trinei;M. Russo;C. Gualerzi
本发明描述了经N-甲基-N '-硝基-N-亚硝基胍（MNNG）诱变和紫外线照射后产生对8-氮鸟嘌呤或β-（2-噻吩基）-DL-丙氨酸具有抗性的钝顶螺旋藻细胞。通过监测细胞活力和两种类型突变体的出现来确定诱变的条件，所述突变体的出现是三体生长阶段和处理的长度和条件的函数。紫外线和MNNG诱变的最佳条件被认为是1-3分钟的照射和50微克MNNG/ml的tricomes来自于培养物进入稳定期分别超声处理10秒和5秒孵育30分钟。在这些条件下，在UV和MNNG诱变后，β-（2-噻吩基）-DL-丙氨酸抗性突变体分别以大于或等于10（-4）和大于或等于10（-5）的频率出现。8-氮鸟嘌呤抗性突变体被发现的频率约。10（-5）。经紫外线处理后，也获得了一些抗氯酸盐的突变体。
The production of Spirulina platensis cells resistant to 8-azaguanine or beta-(2-thienyl)-DL-alanine following mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and UV-irradiation is described. The conditions for the mutagenesis were determined by monitoring cell viability and the appearance of the two types of mutants as a function of the stage of growth of the tricomes and the length and the conditions of the treatment. The optimal conditions for UV and MNNG mutagenesis were found to be 1-3 min irradiation and 30 min incubation with 50 micrograms MNNG/ml of tricomes derived from cultures entering stationary phase sonicated for 10 s and 5 s respectively. Under these conditions beta-(2-thienyl)-DL-alanine-resistant mutants appeared at a frequency greater than or equal to 10(-4) and greater than or equal to 10(-5) following UV- and MNNG-mutagenesis, respectively. Mutants resistant to 8-azaguanine were found at a frequency approx. 10(-5) only after MNNG mutagenesis. A few chlorate-resistant mutants were also obtained following UV treatment.