Tissue-engineered bone regeneration
Tissue-engineered bone regeneration
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DOI：
10.1038/79449
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发表时间：
2000-09-01
影响因子：
46.9
通讯作者：
Guillemin, G
中科院分区：
文献类型：
--
作者：
Petite, H;Viateau, V;Guillemin, G
超过临界尺寸的骨损伤会留下疤痕而不是再生，导致骨不连。我们试图通过使用具有再生能力的细胞的可吸收支架来获得更大程度的再生，以在受伤的成体组织中重建胚胎环境，从而改善临床结果，我们使用珊瑚支架与体外扩增的骨髓基质细胞（MSC）的组合来增加成骨作用，比单独使用支架或支架加新鲜骨髓所获得的效果更多，在绵羊的大节段缺损模型中评估了各种组合的效率，组织工程人造骨进行了在最有利的情况下，形态发生导致完全再皮质化并形成具有成熟板层皮质骨的髓管。当缺损留空或仅用支架填充时，从未发生临床愈合。相反，当缺损用组织工程骨填充时，七分之三的手术肢体获得了临床愈合。
Bone lesions above a critical size become scarred rather than regenerated, leading to nonunion. We have attempted to obtain a greater degree of regeneration by using a resorbable scaffold with regeneration-competent cells to recreate an embryonic environment in injured adult tissues, and thus improve clinical outcome, We have used a combination of a coral scaffold with in vitro-expanded marrow stromal cells (MSC) to increase osteogenesis more than that obtained with the scaffold alone or the scaffold plus fresh bone marrow, The efficiency of the various combinations was assessed in a large segmental defect model in sheep, The tissue-engineered artificial bone underwent morphogenesis leading to complete recorticalization and the formation of a medullary canal with mature lamellar cortical bone in the most favorable cases. Clinical union never occurred when the defects were left empty or filled with the scaffold alone, In contrast, clinical union was obtained in three out of seven operated limbs when the defects were filled with the tissue-engineered bone.