THE STRUCTURAL AND FUNCTIONAL-ORGANIZATION OF THE MURINE HOX GENE FAMILY RESEMBLES THAT OF DROSOPHILA HOMEOTIC GENES
THE STRUCTURAL AND FUNCTIONAL-ORGANIZATION OF THE MURINE HOX GENE FAMILY RESEMBLES THAT OF DROSOPHILA HOMEOTIC GENES
复制标题
DOI：
10.1002/j.1460-2075.1989.tb03534.x
复制
发表时间：
1989-05-01
期刊：
影响因子：
11.4
通讯作者：
DOLLE, P
中科院分区：
文献类型：
--
作者：
DUBOULE, D;DOLLE, P
本文报告了第四主要的鼠雄烯综合体Hox-5的克隆。该基因簇的部分表征揭示了位于该复合物的5''末端的两个新基因（HOX-5.2，HOX-5.3）的存在。原位杂交实验表明，在胚胎和胎儿发育过程中，这两个基因在非常后域中转录。我们还表明，位于HOX-1复合物中最多位置的基因HOX-1.6在早期发育过程中具有非常前表的边界。这些结果清楚地支持了最近提出的假设，即沿着前后发育的身体轴的鼠类抗抗抗抗苯基的表达遵循了一个位置层次，这反映了它们在HOX簇中的各自的物理位置，类似于发现的物理位置。果蝇同源基因。在大多数脊椎动物中，这种结构和功能组织可能是保守的。此外，在序列比较的基础上，我们建议果蝇和室内小鼠之间保守了簇中含同源物的基因的顺序。因此，在昆虫和脊椎动物中，通过进化保守的基因网络可能会显示出类似的调节性相互作用，可以实现非常不同的身体计划。
This paper reports the cloning of the fourth major murine homeogene complex, HOX-5. The partial characterization of this gene cluster revealed the presence of two novel genes (Hox-5.2, Hox-5.3) located at the 5'' extremity of this complex. In situ hybridization experiments showed that these two genes are transcribed in very posterior domains during embryonic and foetal development. We also show that Hox-1.6, the gene located at the 3'' most position in the HOX-1 complex, has a very anterior expression boundary during early development. These results clearly support the recently proposed hypothesis that the expression of murine Antp-like homeobox-containing genes along the antero-posterior developing body axis follows a positional hieracrchy which reflects their respective physical positions within the HOX clusters, similar to that which is found for the Drosophila homeotic genes. Such a structural and functional organization is likely conserved in most vertebrates. Moreover, on the basis of sequence comparisons, we propose that the ordering of homeobox-containing genes within clusters has been conserved between Drosophila and the house mouse. Thus, very different body plans might be achieved, both in insects and vertebrates, by evolutionarily conserved gene networks possibly displaying similar regulatory interactions.