STABILITY OF HEPATITIS-A VIRUS
STABILITY OF HEPATITIS-A VIRUS
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DOI：
10.1159/000149554
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发表时间：
1984-01-01
期刊：
影响因子：
4.6
通讯作者：
KRONAUER, G
中科院分区：
文献类型：
--
作者：
SIEGL, G;WEITZ, M;KRONAUER, G
在严格控制的相同pH值、温度和盐浓度条件下，比较了甲型肝炎病毒（HAV）和2型脊髓灰质炎病毒的稳定性。虽然从pH 3到11，病毒的抗性是相同的，但50%的脊髓灰质炎病毒颗粒在pH 7.0加热10分钟（T50,10 = 43度）时崩解的温度与甲肝病毒（T50,10 = 61度）的特性有显著差异。在1 M MgCl2存在时，脊髓灰质炎病毒和甲肝病毒的t50,10转移到61度。分别是81度。加热破坏HAV的物理完整性导致病毒RNA的释放，同时产生几个空衣壳结构或其解离产物。空衣壳和进一步解离产物仍然与人恢复期血清中含有的抗甲型肝炎IgG具有血清学反应。进一步加热（> T50,10 = 67度）。（> T50,1 = 87度），然而，它们不可逆地失去了这种反应性。
The stabilities of hepatitis A virus (HAV) and of poliovirus type 2 were compared under strictly controlled, identical conditions of pH value, temperature and salt concentration. Although the resistance of the viruses proved to be the same from pH 3 to 11, the temperature at which 50% of poliovirus particles became disintegrated during heating at pH 7.0 for 10 min (T50,10 = 43.degree.) differed significantly from that characteristic for HAV (T50,10 = 61.degree.). In the presence of 1 M MgCl2, the T50,10 for poliovirus and for HAV shifted to 61.degree. and 81.degree., respectively. Destabilization of the physical integrity of HAV by heating resulted in the release of viral RNA and, simultaneously, in the generation of several empty capsid structures or dissociation products thereof. Empty capsids and further dissociation products were still serologically reactive with anti-HAV IgG contained in human convalescent sera. With further heating (> T50,10 = 67.degree. or > T50,1 = 87.degree.), however, they irreversibly lost this reactivity.