Bacterial population dynamics in three anopheline species: The impact on Plasmodium sporogonic development
Bacterial population dynamics in three anopheline species: The impact on Plasmodium sporogonic development
复制标题
DOI：
10.4269/ajtmh.1996.54.214
复制
发表时间：
1996-02-01
影响因子：
3.3
通讯作者：
Beier, JC
中科院分区：
文献类型：
--
作者：
Pumpuni, CB;DeMaio, J;Beier, JC
细菌在成年蚊子中肠的功能作用尚不清楚。本文研究了实验室饲养的斯氏按蚊、阿氏按蚊和中华按蚊中肠细菌的种群动态。gambiae和An. albimanus。在无菌条件下解剖苔藓中肠，并使用标准微生物学技术检查细菌的存在。90%和73%（n = 30）的新出现的An。gambiae和An. stephensi，分别窝藏细菌。与此相反，只有17%（n = 23）的AN。阿尔比曼纳斯窝藏任何细菌。在血餐的存在下，细菌数量增加了11-40倍，但随后在3-5天内下降到血餐前的水平。洋葱假单胞菌、成团肠杆菌和黄杆菌属。在这三种按蚊身上都有发现中肠细菌通过经食道和通过糖餐获得。通过成功地将大肠杆菌HS 5从幼虫阶段传代至成虫阶段，证明了经口传播。然而，中肠细菌获得更有效地通过糖餐比通过transstadial通道。蚊子暴露于细菌/糖悬浮液后中肠细菌计数的增加显着降低了恶性疟原虫感染蚊子队列中的卵囊感染率和密度。由于细菌在野生蚊子中天然存在，因此可以使用引入的或固有的细菌来修饰按蚊载体感受态。
The functional role of bacteria in the midgut of adult mosquitoes is unknown. In this study, we examined the population dynamics of midgut bacteria of laboratory reared Anopheles stephensi, An. gambiae, and An. albimanus. Mosquito midguts were dissected under sterile conditions and examined for the presence of bacteria using standard microbiologic techniques. Ninety percent and 73% (n = 30) of newly emerged An. gambiae and An. stephensi, respectively, harbored bacteria. In contrast, only 17% (n = 23) of An. albimanus harbored any bacteria. The bacterial population increased 11-40-fold in the presence of a blood meal, but then decreased to pre-blood meal levels in 3-5 days. Pseudomonas cepacia, Enterobacter agglomerans, and Flavobacterium spp. were found in all three anopheline species. Midgut bacteria were acquired both transtadially and through the sugar meal. Transtadial transmission was demonstrated by successfully passaging Escherichia coli HS5 from the larval to the adult stage. However, midgut bacteria were acquired more efficiently through the sugar meal than through transtadial passage. An increase in midgut bacterial counts after mosquitoes were exposed to a bacteria/sugar suspension significantly reduced oocyst infection rates and densities in Plasmodium falciparum-infected mosquito cohorts. Since bacteria occur naturally in wild mosquitoes, it may be possible to modify anopheline vector competence using introduced or indigenous bacteria.