Development and Use of an ELISA Test to Detect IgE Antibody to Cry9c following Possible Exposure to Bioengineered Corn
Development and Use of an ELISA Test to Detect IgE Antibody to Cry9c following Possible Exposure to Bioengineered Corn
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开发和使用 ELISA 测试来检测可能接触生物工程玉米后的 Cry9c IgE 抗体
DOI：
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发表时间：
2003
影响因子：
2.8
通讯作者：
Carol Rubin
中科院分区：
文献类型：
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作者：
R. Raybourne;K. Williams;R. Vogt;Dori B. Reissman;B. Winterton;Carol Rubin
背景：Starlink™是一种经过基因工程改造含有杀虫蛋白Cry9c的玉米品种，由于其具有一些与致敏蛋白相关的特性，未被批准用于人类食用。然而，在2000年秋季，在几种含玉米的产品中检测到了Cry9c DNA，这表明Starlink玉米已进入人类食品供应环节。随后，消费者在食用玉米产品后报告了一些不良健康事件，可能与过敏反应相符。
方法：为了调查这些消费者中因Cry9c产生过敏反应的可能性，开发了一种酶联免疫吸附测定（ELISA）试验，用于检测针对Cry9c的IgE抗体，并从总共18名自述有过敏反应的人身上采集了血液样本。将1996年Starlink研发之前采集的血清用作阴性对照。
结果：根据与正常对照的比较，未发现不良事件血清与重组Cry9c抗原发生反应。尽管没有已知的含有针对Cry9c的特异性IgE的人类阳性对照血清，但在ELISA方案中纳入了其他对照，包括使用对其他过敏原及其同源抗原（猫、草、花生）过敏的受试者的血清来验证IgE检测试剂。
结论：虽然结果不支持对Cry9c发生过敏反应的可能性，但不能排除此类反应，也不能排除血清可能与玉米植株/种子中可能表达的Cry9c的独特糖基化表位发生反应的可能性。
Background: StarlinkTM, a variety of corn genetically engineered to contain the insecticidal protein Cry9c, had not been approved for human consumption because it possessed some characteristics associated with allergenic proteins. However, in the fall of 2000 cry9c DNA was detected in several corn-containing products, suggesting that Starlink corn had entered the human food supply. Subsequently, consumers, following consumption of corn products, reported a number of adverse health events, possibly consistent with allergic reaction. Methods: To investigate the possibility of allergic reactions due to Cry9c in these consumers an ELISA test was developed for the purpose of detecting IgE antibodies to Cry9c and blood samples were taken from a total of 18 people who self-reported allergic reactions. Sera collected prior to the 1996 development of Starlink were used as negative controls. Results: None of the adverse event sera were found to be reactive with recombinant Cry9c antigen, based on comparison with normal controls. Although a known human positive control serum containing IgE specific for Cry9c was not available, other controls were incorporated into the ELISA protocol, including the use of sera from subjects allergic to other allergens and their homologous antigens (cat, grass, peanut) to validate the IgE detection reagents. Conclusions: While the results do not support the likely occurrence of allergic reactions to Cry9c, such reactions cannot be ruled out, nor can the possibility that sera might react with unique glycosylated epitopes of Cry9c that may be expressed in the corn plant/seed.
虾特异性 IgG 亚类抗体与对虾攻击的直接不良反应之间的关系。
DOI：
10.1016/s0091-6749(05)80102-2
发表时间：
1990
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Morgan,JE;Daul,CB;Lehrer,SB
通讯作者：
Lehrer,SB