Blue‐green fluorescence excited by UV laser on leaves of different species originates from cutin and is sensitive to leaf temperature
Blue‐green fluorescence excited by UV laser on leaves of different species originates from cutin and is sensitive to leaf temperature
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不同物种叶片上紫外激光激发的蓝绿色荧光来源于角质，对叶片温度敏感
DOI：
10.1111/j.1365-3040.1994.tb00171.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Y. Goulas
中科院分区：
文献类型：
--
作者：
G. Bongi;A. Palliotti;P. Rocchi;I. Moya;Y. Goulas
在紫外线的激发下，完整的叶片产生强烈的蓝绿荧光发射，并具有多个波段。它们的综合能量是叶绿素a波段释放能量的6 - 11倍（Chappelle et al. 1984, Applied Optics 23,134 - 138）。本文提供的证据表明，蓝绿色荧光发射主要来自叶片的外表皮层，并可以通过将叶片快速浸入有机溶剂中并随后溶剂蒸发而转移到石英片上。蓝绿荧光在4°C（高荧光）和37°C（低荧光）之间显示扩散控制的荧光强度猝灭。蓝绿色荧光发射率与叶片温度以外的短期代谢效应无关，但表皮对干旱和过度辐射的适应都增加了发射率。
Under ultra-violet excitation, intact leaves generate a strong blue-green fluorescence emission with several bands. Their integrated energy is 6 to 11 times the energy released by chlorophyll a bands (Chappelle et al. 1984, Applied Optics 23, 134–138). This paper provides evidence that the blue-green fluorescence emission comes mainly from outer epidermal layers of the leaves and can be transferred on a quartz lamina by quickly dipping the leaves in organic solvents with subsequent solvent evaporation. Blue-green fluorescence displays a diffusion-controlled quenching of fluorescence intensity between 4°C (high fluorescence) and 37°C (low fluorescence). The blue-green fluorescence emissivity is not linked to short-term metabolic effects other than leaf temperature, but epidermis adaptations both to drought and to excessive radiation increase emissivity.