Flow cytometric analysis of bacterial respiratory and enzymatic activity in the natural aquatic environment
Flow cytometric analysis of bacterial respiratory and enzymatic activity in the natural aquatic environment
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自然水生环境中细菌呼吸和酶活性的流式细胞术分析
DOI：
10.1046/j.1365-2672.1997.00165.x
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发表时间：
1997
影响因子：
4
通讯作者：
M. Nasu
中科院分区：
文献类型：
--
作者：
N. Yamaguchi;M. Nasu
采用流式细胞仪研究了污染河水和未污染河水中细菌的呼吸活性和酶活性。使用6CFDA（6-羧基荧光素二乙酸酯）和PI（碘化丙啶）双重染色检查细菌酯酶活性。采用CTC（5-氰基-2，3-二甲苯基四唑氯化物）作为细菌呼吸的指示剂。除高污染场所外，菌落形成单位占细菌总数的比例均小于2%。用流式细胞仪测定的呼吸细菌数量在未污染和污染的采样点均占细菌总数的10-15%，而在污染严重的采样点则占30%。近50%的总细菌表现出酯酶活性在未污染的地区，而70-90%的总细菌保留这种酶活性在污染的地区。因此，无论污染程度如何，自然水环境中的许多不可培养细菌都具有酶活性，而6 CFDA在评价自然环境中细菌生理活性的灵敏度方面更上级。具有酯酶活性的细菌的比例与河水的污染程度成正比，这表明该比例是一个有用的指标，在水环境中的污染程度进行评估。
The respiratory and enzymatic activity of bacteria in polluted and unpolluted river water was investigated by flow cytometry. Double staining with 6CFDA (6‐carboxy fluorescein diacetate) and PI (propidium iodide) was used to examine bacterial esterase activity. CTC (5‐cyano‐2,3‐ditolyl tetrazolium chloride) was employed as the indicator of bacterial respiration. The ratios of colony‐forming units to total bacterial number were less than 2%, except highly polluted sites. The number of respiring bacteria determined by flow cytometry amounted to 10–15% of the total bacterial number at both unpolluted and polluted sampling stations, while it was 30% at the highly polluted station. Almost 50% of total bacteria demonstrated esterase activity in the unpolluted areas, whereas 70–90% of total bacteria retained this enzymatic activity in the polluted areas. Thus, many of the non‐culturable bacteria in the natural aquatic environment have enzymatic activity regardless of the pollution level, and 6CFDA was more superior in the sensitivity for the evaluation of physiological activity of bacteria in the natural environment. The ratio of bacteria with esterase activity increased in direct proportion to the pollution level of river water and suggested that this ratio would be a useful indicator in evaluating the pollution levels in an aquatic environment.