Light, temperature, and aging dependent vegetative growth and sporulation of Colletotrichum gloeosporioides on different culture media
Light, temperature, and aging dependent vegetative growth and sporulation of Colletotrichum gloeosporioides on different culture media
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DOI：
10.5897/jmpr12.1308
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发表时间：
2014-01
影响因子：
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通讯作者：
J. Soltani;M. Haghighi;S. Nazeri
中科院分区：
文献类型：
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作者：
J. Soltani;M. Haghighi;S. Nazeri
炭疽菌是引起柑橘炭疽病的病原菌。它最近被认为是一种潜在的抗癌代谢物紫杉醇的生产商。在这里，我们介绍了球孢链霉菌生长和产孢子的最佳条件。以马铃薯葡萄糖琼脂(PDA)、康乃馨叶片琼脂(CLA)、马铃薯胡萝卜琼脂(PCA)和水琼脂(WA)为基础，添加Watman或Fabriano滤纸等产孢剂，并对其营养生长和产孢量进行了评价。3种光照方案，即连续光照、16/8小时光照/黑暗和连续黑暗与培养基相结合。分别于孵化后第7、15、21、30天进行跟踪观察。在28℃的连续光照条件下，PDA和PCA培养基能为球孢霉的最大生长提供最佳条件。光照周期的缩短降低了真菌的生长。此外，真菌产孢量对光照、温度和培养基有很高的依赖性。在相同温度下，在16/8h的光照/黑暗时间间隔下，球孢霉在放置在PDA培养基上的Fabriano纸上产孢量最大。在较低的温度下，即22℃时，球孢霉在同一培养基上的产孢量极低。此外，老化通常会增加真菌产孢量。关键词：炭疽菌，柑橘，生长，分生，发育。
The fungal organism Colletotrichum gloeosporioides is the causative agent of anthracnose disease of Citrus fruits. It is recently introduced as a potential producer of anticancer metabolite paclitaxel. Here, we introduce the optimal conditions for growth and sporulation of C. gloeosporioides. We have considered four fungal culture media, that is potato dextrose agar (PDA), carnation leaf agar (CLA), potato carrot agar (PCA) and water agar (WA), based on which sporulation inducers like Watman or Fabriano filter papers could be added, and evaluated both for vegetative growth and sporulation. Three light regimens, i.e. continuous light, 16/8 hrs light/darkness, and continuous darkness were applied in combination with the culture media. All experiments were tracked on 7th, 15th, 21st, and 30th day after incubation. At 28°C, PDA and PCA culture media, under continuous light, provided the best condition for C. gloeosporioides maximal growth. Decreasing light periods decreased the fungal growth. Furthermore, fungal sporulation showed a high dependence on light, temperature and culture medium in use. Under 16/8 h light/darkness interval at the same temperature C. gloeosporioides sporulation was at its maximum on Fabriano paper placed on PDA medium. At a lower temperature, that is 22°C, C. gloeosporioides sporulation on the same culture media was highly defected. Furthermore, aging generally increased the fungal sporulation. Key words: Colletotrichum gloeosporioides, citrus, growth, conidiation, development.