Evaluation of in vitro secondary caries using confocal laser scanning microscope and X-ray analytical microscope.
Evaluation of in vitro secondary caries using confocal laser scanning microscope and X-ray analytical microscope.
复制标题
使用共焦激光扫描显微镜和X射线分析显微镜评价体外继发龋。
DOI：
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复制
发表时间：
2003
影响因子：
1.4
通讯作者：
J. Tagami
中科院分区：
文献类型：
--
作者：
M. Okuda;P. Pereira;T. Nikaido;J. Tagami
目的
采用激光共聚焦扫描显微镜（CLSM）评价离体牙继发龋的形成和抑制情况，并进一步用X射线分析显微镜（XAM）绘制钙分布图。
材料和方法
在牛牙根牙本质上制备36个盒形洞，并使用Fuji II、Fuji II LC（改良）或Clearfil Liner Bond 2 V系统（含非氟释放树脂基复合材料）进行修复; Clearfil AP-X用作阴性对照。在50 mmol/L乙酸缓冲去矿物质溶液中浸泡3.5天后，将pH值调节至4.5，通过每个修复体切割纵向半部分，并抛光，用于CLSM下的分析。为了确认CLSM结果，随后通过X射线分析显微镜（XAM）检查相同的样品。通过两种不同的CLSM和XAM测量获得的外部病变和抑制区的值然后通过单因素ANOVA、Fisher PLSD检验在95%置信水平和相关系数下进行分析。
结果
在CLSM下，玻璃离子和树脂改性玻璃离子涂层周围的抑制区和外部病变被清楚地检测到。正如预期的那样，在树脂基复合材料修复体附近未检测到抑制区。此外，使用XAM的透射X射线图像，观察到抑制区为高密度区，外部病变为低密度区。在CLSM下测量的抑制区的宽度和外部损伤的深度与从XAM获得的那些相似。
PURPOSE
To evaluate in vitro secondary caries formation and inhibition by using a confocal laser scanning microscope (CLSM), and further map the calcium distribution with an X-ray analytical microscope (XAM).
MATERIALS AND METHODS
36 box-shaped cavities were prepared on bovine root dentin and restored with Fuji II, Fuji II LC (improved), or Clearfil Liner Bond 2V system with a non-fluoride releasing resin-based composite; Clearfil AP-X was used as a negative control. After immersion in a buffered demineralizing solution of 50 mmol/L of acetic acid adjusted to pH 4.5 for 3.5 days, longitudinal half sections were cut through each restoration and polished for analysis under CLSM. In order to confirm the CLSM results, the same samples were subsequently examined by means of the X-ray analytical microscope (XAM). The values of the outer lesions and the inhibition zones obtained by the two different CLSM and XAM measurements were then analyzed by one-way ANOVA, Fisher's PLSD Test at the 95% level of confidence and correlation coefficient.
RESULTS
Inhibition zones and outer lesions were clearly detected under CLSM around the glass-ionomer and resin-modified glass-ionomer restorations. As expected, an inhibition zone was not detected adjacent to the resin-based composite restoration. Additionally, using the transmission x-ray images of XAM, the inhibition zones were observed as high density areas, and the outer lesions as low density areas. The width of the inhibition zones and the depth of the outer lesions measured under the CLSM were similar to those obtained from the XAM.