Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice
Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in rice
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DOI：
10.1111/j.1365-313x.2005.02428.x
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发表时间：
2005-07-01
期刊：
影响因子：
7.2
通讯作者：
Kumagai, T
中科院分区：
文献类型：
--
作者：
Hidema, J;Teranishi, M;Kumagai, T
不同水稻品种对UVB(280-320 nm)辐射的敏感性差异很大。我们以前曾指出，抗紫外线水稻品种比紫外线敏感品种更能通过光修复修复环丁烷嘧啶二聚体(CPD)。在这篇文章中，我们报道了水稻对UVB的敏感性，部分是CPD光解酶等位基因缺陷的结果。与抗紫外线的早稻相比，苏拉米吉对UVB辐射表现出更高的敏感性，CPD光修复能力更差。Surjamkhi的CPD光修复缺陷是由于光解酶基因第377和888位的两个核苷酸发生变化，导致光解酶126和296位的两个推导氨基酸发生变化。来自Surjamkhi和Sasanishiki的群体的连锁分析表明，UVB敏感性是一个数量遗传性状，CPD光解酶基因座与一个数量性状基因座紧密连锁，该基因座解释了该性状的大部分遗传变异。这些结果表明，CPD光解酶基因的自发突变导致水稻对UVB具有不同程度的敏感性，通过常规育种或生物工程提高光解酶的功能可以提高水稻对UVB辐射的抗性。
Sensitivity to ultraviolet-B (UVB) radiation (280-320 nm) varies widely among rice cultivars. We previously indicated that UV-resistant rice cultivars are better able to repair cyclobutane pyrimidine dimers (CPDs) through photorepair than are UV-sensitive cultivars. In this paper, we report that UVB sensitivity in rice, in part, is the result of defective CPD photolyase alleles. Surjamkhi (indica) exhibited greater sensitivity to UVB radiation and was more deficient in CPD photorepair ability compared with UV-resistant Sasanishiki (japonica). The deficiency in CPD photorepair in Surjamkhi resulted from changes in two nucleotides at positions 377 and 888 in the photolyase gene, causing alterations of two deduced amino acids at positions 126 and 296 in the photolyase enzyme. A linkage analysis in populations derived from Surjamkhi and Sasanishiki showed that UVB sensitivity is a quantitative inherited trait and that the CPD photolyase locus is tightly linked with a quantitative trait locus that explains a major portion of the genetic variation for this trait. These results suggest that spontaneously occurring mutations in the CPD photolyase gene cause different degrees of sensitivity to UVB in rice, and that the resistance of rice to UVB radiation could be increased by increasing the photolyase function through conventional breeding or bioengineering.