Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.
Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells.
复制标题
SLIT2在排斥和增强躯干的迁移中的双重功能，而不是迷走神经rest细胞。
DOI：
10.1083/jcb.200301041
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发表时间：
2003-07-21
影响因子：
7.8
通讯作者：
Bronner-Fraser, Marianne
中科院分区：
文献类型：
--
作者：
De Bellard, Maria Elena;Rao, Yi;Bronner-Fraser, Marianne
自主神经系统的神经嵴前体根据其轴向起源水平形成不同的衍生物;例如，迷走神经而不是躯干神经嵴细胞形成肠道的肠神经节。在这里，我们表明，Slit 2是在入口处的肠道，这是选择性地入侵迷走神经，但不是躯干，神经嵴。因此，只有躯干神经嵴细胞表达Robo受体。体内和体外实验表明，躯干，而不是迷走神经，嵴细胞避免表达Slit 2的细胞或细胞膜，从而有助于神经嵴群体的分化能力，侵入和神经支配肠道。相反，暴露于可溶性Slit 2显著增加了躯干神经嵴细胞穿过的距离。这些结果表明，Slit 2可以发挥双功能，既排斥和刺激躯干神经嵴细胞的运动。
Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.