Reduced expression of retinoic acid receptor beta protein (RARβ) in human papillary thyroid carcinoma:: immunohistochemical and Western blot study
Reduced expression of retinoic acid receptor beta protein (RARβ) in human papillary thyroid carcinoma:: immunohistochemical and Western blot study
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DOI：
10.1046/j.1365-2559.1998.00486.x
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发表时间：
1998-10-01
期刊：
影响因子：
6.4
通讯作者：
Jozan, S
中科院分区：
文献类型：
--
作者：
Rochaix, P;Monteil-Onteniente, S;Jozan, S
目的：为了研究视黄酸受体β（RARβ）在甲状腺癌发生中的作用，我们通过免疫组织化学和蛋白质印迹相结合的方法研究了其在人甲状腺样本中的表达。方法和结果：使用特异性单克隆抗体对58个石蜡包埋的甲状腺样本（40个正常或良性组织，16个乳头状癌和2个滤泡状癌）进行了免疫组织化学分析。还对 11 个选定的样本（7 个正常或良性组织、3 个乳头状癌和 1 个滤泡癌）和 2 个人类卵巢癌作为对照进行了蛋白质印迹分析。 RAR β 免疫染色是核染色，仅限于正常上皮甲状腺组织。在所有乳头状癌和一种滤泡性癌中观察到 RAR β 免疫染色显着降低。另一种滤泡癌表现出强烈的 RAR β 免疫染色。通过免疫印迹，在正常甲状腺和一种滤泡癌的核提取物中观察到与 RAR β 相对应的 51 kDa 信号。然而，乳头状癌中缺乏这种信号。这些结果与通过免疫组织化学对相同样品获得的观察结果完全一致。结论：我们在此首次证明正常人甲状腺滤泡细胞中存在 RAR β 蛋白。此外，我们发现其表达在甲状腺乳头状癌中降低。
Aims: To investigate the role of retinoic acid receptor beta (RAR beta) in thyroid carcinogenesis, we have investigated its expression in human thyroid samples by combined immunohistochemistry and Western blotting.Methods and results: Fifty-eight paraffin-embedded thyroid samples (40 normal or benign tissues, 16 papillary and two follicular carcinomas) were analysed by immunohistochemistry using a specific monoclonal antibody. Western blotting was also carried out on 11 selected samples (seven normal or benign tissues, three papillary carcinomas and one follicular carcinoma) and two human ovarian carcinomas as controls. RAR beta immunostaining was nuclear and limited to the normal epithelial thyroid tissue. A dramatic decrease in RAR beta immunostaining was observed in all the papillary carcinomas and in one follicular carcinoma. The other follicular carcinoma exhibited strong RAR beta immunostaining, By immunoblotting, a 51 kDa signal corresponding to the RAR beta was observed in nuclear extracts from normal thyroids and for one follicular carcinoma. However, this signal was lacking in the papillary carcinomas. These results were in complete agreement with the observations obtained by immunohistochemistry on the same samples.Conclusion: We present here the first demonstration of RAR beta protein in normal human thyroid follicular cells. In addition, we found that its expression is decreased in papillary thyroid carcinoma.