Immortalized B lymphocytes produce B-cell growth factor
Immortalized B lymphocytes produce B-cell growth factor
复制标题
永生化 B 淋巴细胞产生 B 细胞生长因子
作者：
J. Gordon;S. Ley;M. Melamed;L. English;N. Hughes
由抗原（或其受体的抗体）引发的小静息B淋巴细胞的活化、克隆扩增和终末分化似乎遵循由不同的可溶性细胞因子（主要由T淋巴细胞产生）在每个阶段触发的有序发育序列1，2。对于人类，唯一已知的不依赖于辅助细胞的B细胞有丝分裂原是EB病毒（EBV）。由EBV转化的淋巴细胞从B细胞生长的通常限制中释放出来，在没有任何外源性精氨酸的情况下持续增殖3。所得到的细胞系是特别感兴趣的，因为它们具有与伯基特淋巴瘤的癌前状态相容的某些特征，伯基特淋巴瘤是与病毒密切相关的两种人类癌症之一3。我们在此报道，EBV转化后，B淋巴母细胞释放一种可溶性因子，该因子模拟丝裂原条件T淋巴细胞的B细胞刺激产物。此外，病毒转化的细胞利用这种活性来维持其自身的生长。异位生产，否则正常的生长因子可能代表一个关键事件，在恶性演变的人类淋巴瘤窝藏的EBV基因组。
The activation, clonal expansion and terminal differentiation of small resting B lymphocytes primed by an antigen (or antibodies to its receptors) appear to follow an orderly developmental sequence triggered at each stage by distinct soluble cytokines, primarily produced by T lymphocytes1,2. For man, the only known B-cell mitogen independent of accessory cells for its action is the Epstein–Barr virus (EBV). Lymphocytes transformed by EBV are released from the usual constraints on B-cell growth, proliferating continuously in the absence of any exogenous cytokine3. The resultant cell lines are of special interest as they possess certain features compatible with a preneoplastic state of Burkitt's lymphoma, one of two human cancers with which the virus is intimately associated3. We report here that following EBV-transformation, B lympho-blasts release a soluble factor which mimics the B-cell stimulatory product(s) of mitogen-conditioned T lymphocytes. Furthermore, the virally-transformed cells utilize this activity to sustain their own growth. The ectopic production of an otherwise normal growth factor may represent a critical event in the malignant evolution of human lymphomas harbouring the EBV genome.
由人类恶性 T 淋巴细胞系产生的白血病衍生生长因子（非白细胞介素 2）。
DOI：
10.1073/pnas.79.22.7004
发表时间：
1982
影响因子：
11.1
作者：
Uittenbogaart,CH;Fahey,JL
通讯作者：
Fahey,JL