DNA damage in BV-2 cells: An important supplement to the neurotoxicity of CdTe quantum dots
DNA damage in BV-2 cells: An important supplement to the neurotoxicity of CdTe quantum dots
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BV-2细胞中的DNA损伤：CdTe量子点神经毒性的重要补充
DOI：
10.1002/jat.3745
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发表时间：
2019-03-01
影响因子：
3.3
通讯作者：
Tang, Meng
中科院分区：
文献类型：
--
作者：
He, Keyu;Liang, Xue;Tang, Meng
小胶质细胞是中枢神经系统中的常驻免疫细胞。由CdTe量子点（QD）诱导的小胶质细胞的激活可触发对神经元的损伤。为了定量细胞内的量子点，我们监测了量子点暴露的小鼠小胶质细胞（BV-2细胞系）的细胞内镉浓度。不同时间的细胞损伤程度与细胞内镉浓度有关。除了镉离子检测，我们还监测了量子点的细胞内荧光。在细胞核中积累的量子点多于细胞质。彗星实验证实量子点诱导DNA损伤。但由于量子点不能与DNA相互作用，因此DNA的损伤不是由量子点与DNA的加合物引起的，而是由Cd离子浓度的增加和二次氧化损伤引起的。除了DNA损伤，生物膜损伤和内源性还原型谷胱甘肽耗竭也明显在QD暴露的BV-2细胞。这些变化可以通过外源性还原型谷胱甘肽给药来预防甚至逆转。
Microglial cells are resident immune cells in the central nervous system. Activation of microglia as induced by CdTe quantum dots (QDs) can trigger damage to neurons. To quantify the intracellular QDs, we monitored the intracellular Cd concentration in the QD-exposed mouse microglial cells (BV-2 cell line). The extent of cell injury at different times correlated with the Cd concentration in cells at that time. In addition to Cd ion detection, we also monitored the intracellular fluorescence of the QDs. More QDs accumulated in the nucleus than in the cytoplasm. Comet assays confirmed that QDs induce DNA damage. However, DNA cannot interact with QDs, so the DNA damage was not caused by CdTe QDs adducts to DNA but by the increase of the Cd ion concentration and the secondary oxidative damage. In addition to DNA damage, biofilm injury and endogenous reduced glutathione depletion were also apparent in QD-exposed BV-2 cells. These changes can be prevented or even reversed by exogenous reduced glutathione administration.