Vascular leukocytes contribute to tumor vascularization
Vascular leukocytes contribute to tumor vascularization
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DOI：
10.1182/blood-2004-05-1906
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发表时间：
2005-01-15
期刊：
影响因子：
20.3
通讯作者：
Coukos, G
中科院分区：
文献类型：
--
作者：
Conejo-Garcia, JR;Buckanovich, RJ;Coukos, G
没有证据表明造血细胞对出生后的血管发生有显着贡献。在这里，我们报告了卵巢癌中一种新的白细胞亚群，它共表达内皮细胞和树突状细胞标记物。荧光激活细胞分选仪 (FACS) 分析发现，在评估的 10 个实体瘤中有 10 个中，VE-钙粘蛋白 (+) CD45(+) 白细胞（占宿主细胞的 39%）的频率较高。该细胞群占腹水和外周血中非肿瘤细胞的比例不到 1%。在蛋白质水平上，超过 86% 的这些细胞表达内皮标记物 P1H12、CD34 和 CD31 以及白细胞标记物 CD11c 和主要组织相容性复合体 (MHC) 类 It。在 mRNA 水平上，我们检测到了 TEM1、TEM7 和 Thy-1，它们是血管生成内皮的特异性标记物。最后，这个群体有能力在体内产生功能性血管。由于其混合表型。我们将这个群体命名为血管白细胞（VLC）。我们的数据提供了造血内皮前体细胞与出生后血管发育之间的重要联系以及可能的新治疗靶点。
There is no proof that hematopoietic cells contribute significantly to vasculo-genesis in postnatal life. Here we report a novel leukocyte subset within ovarian carcinoma that coexpresses endothelial and dendritic cell markers. Fluorescence-activated cell sorter (FACS) analysis identified a high frequency of VE-cadherin(+) CD45(+) leukocytes (39% of host cells) in 10 of 10 solid tumors evaluated. This population represented less than 1% of nontumor cells in ascites and peripheral blood. At the protein level, more than 86% of these cells expressed the endothelial markers P1H12, CD34, and CD31 and leukocyte markers CD11c and major histocompatibility complex (MHC) class It. At the mRNA level, we detected TEM1, TEM7, and Thy-1, specific markers of angiogenic endothelium. Finally, this population has the capacity to generate functional blood vessels in vivo. Because of its mixed phenotype. we named this population vascular leukocytes (VLCs). Our data provide an important link between hematopoietic endothelial precursors and vascular development in postnatal life and a possible novel therapeutic target.