Microbial population dynamics during startup of a full-scale anaerobic digester treating industrial food waste in Kyoto eco-energy project
Microbial population dynamics during startup of a full-scale anaerobic digester treating industrial food waste in Kyoto eco-energy project
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DOI：
10.1016/j.biortech.2010.01.028
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发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Kadoshin, Shiro
中科院分区：
文献类型：
--
作者：
Ike, Michihiko;Inoue, Daisuke;Kadoshin, Shiro
利用真细菌和古细菌16S rRNA基因的末端限制性片段长度多态性，分析了京都生态能源项目中处理工业餐厨垃圾的全规模厌氧消化器(2300m(3))中的微生物群落。将处理过的猪排泄物的高温和中温污泥接种到消化池中。在150天的启动期间，咖啡渣作为主要的食物垃圾，与土豆、海带和煮豆子、豆腐、豆腐渣、油炸豆腐一起逐步进入消化过程(最大。40t/d)。在停留时间为40d的条件下，甲烷产率达到360m(3)/t，但有丙酸的短暂积累。在高温消化池中形成的真细菌群落与高温和中温类型的种子污泥有很大的不同。结果表明，放线菌/热单孢菌和拉氏杆菌/希瓦氏菌是餐厨垃圾降解为挥发性脂肪酸的贡献者。利用醋酸盐的甲烷菌在这两种类型的种子污泥中占主导地位，但在消化池中加工过程中它们的数量急剧减少。甲烷八叠球菌和甲烷短杆菌/甲烷杆菌分别可能是乙酸酯和H-2+CO2产甲烷的主要贡献者。(C)2010爱思唯尔有限公司。保留所有权利。
The microbial community in a full-scale anaerobic digester (2300 m(3)) treating industrial food waste in the Kyoto Eco-Energy Project was analyzed using terminal restriction fragment length polymorphism for eubacterial and archaeal 16S rRNA genes. Both thermophilic and mesophilic sludge of treated swine waste were seeded to the digestion tank. During the 150-day startup period, coffee grounds as a main food waste, along with potato, kelp and boiled beans, tofu, bean curd lees, and deep-fried bean curd were fed to the digestion process step-by-step (max. 40 t/d). Finally, the methane yield reached 360 m(3)/t-feed with 40 days' retention time, although temporary accumulation of propionate was observed. Eubacterial communities that formed in the thermophilic digestion tank differed greatly from both thermophilic and mesophilic types of seed sludge. Results suggest that the Actinomyces./Thermomonospora and Ralstonia/Shewanella were contributors for hydrolyzation and degradation of food waste into volatile fatty acids. Acetate-utilizing methanogens, Methanosaeta, were dominant in seed sludges of both types, but they decreased drastically during processing in the digestion tank. Methanosarcina and Methanobrevibacter/Methanobacterium were, respectively, possible main contributors for methane production from acetate and H-2 plus CO2. (C) 2010 Elsevier Ltd. All rights reserved.