REACTIVITY OF ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES WITH MONONUCLEAR PHAGOCYTES
REACTIVITY OF ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES WITH MONONUCLEAR PHAGOCYTES
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DOI：
10.1002/jlb.51.1.65
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发表时间：
1992-01-01
影响因子：
5.5
通讯作者：
JENNETTE, JC
中科院分区：
文献类型：
--
作者：
CHARLES, LA;FALK, RJ;JENNETTE, JC
抗中性粒细胞胞浆自身抗体（ANCA）与中性粒细胞颗粒和单核细胞过氧化物酶阳性溶酶体中的蛋白质发生反应，包括髓过氧化物酶（MPO）、蛋白酶3 （PR-3）和弹性酶。anca相关疾病，如Wegener肉芽肿病和结节性多动脉炎，以坏死性血管炎症为特征。炎性病变通常包含中性粒细胞和单核吞噬细胞，后者有时占主导地位，例如Wegener肉芽肿病的肉芽肿病变。我们研究了在体外、肺泡和腹膜巨噬细胞分化过程中单核吞噬细胞细胞质中ANCA靶抗原PR3、MPO和弹性酶的存在。我们观察到，在单核吞噬细胞分化过程中，ANCA抗原被下调，其缺失与过氧化物酶阳性颗粒的缺失相对应。这表明ANCA仅能直接与单核细胞和早期渗出性巨噬细胞相互作用，而不能与成熟巨噬细胞相互作用。
Antineutrophil cytoplasmic autoantibodies (ANCA) react with proteins found in the granules of neutrophils and the peroxidase-positive lysosomes of monocytes, including myeloperoxidase (MPO), proteinase 3 (PR-3), and elastase. ANCA-associated diseases, such as Wegener's granulomatosis and polyarteritis nodosa, are characterized by necrotizing vascular inflammation. The inflammatory lesions typically contain both neutrophils and mononuclear phagocytes, with the latter sometimes predominating, for example, in the granulomatous lesions of Wegener's granulomatosis. We investigated the presence of the ANCA target antigens PR3, MPO, and elastase in mononuclear phagocyte cytoplasm during the course of differentiation in vitro and in alveolar and peritoneal macrophages. We observed that ANCA antigens were down-regulated during mononuclear phagocyte differentiation, with the loss corresponding to that of peroxidase-positive granules. This suggests that ANCA can directly interact only with monocytes and early exudative macrophages and not with mature macrophages.