Intestinal microbiota profiling and predicted metabolic dysregulation in psoriasis patients
Intestinal microbiota profiling and predicted metabolic dysregulation in psoriasis patients
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DOI：
10.1111/exd.13786
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发表时间：
2018-12-01
影响因子：
3.6
通讯作者：
Wu, Chun-Ying
中科院分区：
文献类型：
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作者：
Chen, Yi-Ju;Ho, Hsiu J.;Wu, Chun-Ying
目的肠道微生物区系与肥胖和宿主免疫反应密切相关。我们的目的是研究银屑病患者肠道微生物区系和潜在的遗传功能与临床表现的关系。方法对32例寻常型银屑病患者和年龄、性别、体重指数匹配的非寻常型银屑病患者()的粪便微生物区系和高通量16S核糖体测序预测的遗传功能进行分析。还研究了微生物区系改变与疾病活动性、关节炎和全身抗银屑病药物的相关性。我们观察到银屑病患者有明显的粪便微生物群落结构，在不同的受试者亚组中，细菌门的丰度增加，类杆菌的丰度降低。瘤胃球菌和巨藻是银屑病判别丰度最高的两个属。在银屑病患者的粪便微生物区系中，一些涉及细菌趋化和碳水化合物运输的功能基因和代谢途径被预测为高表达，而与钴胺和铁运输相关的基因被预测为低表达。结论银屑病患者粪便微生物组成的不同可能与碳水化合物、钴胺和铁的转运改变以及趋化性有关。
Objectives The intestinal microbiota has been known to involve in obesity and host immune response. We aimed to investigate the intestinal microbiota and potential genetic function in relation to clinical presentation in psoriasis patients. Methods Results Faecal microbiota and predicted genetic function inferred from high-throughput 16S ribosomal RNA sequencing were analysed between psoriasis (n = 32) and age-, gender- and body mass index (BMI)-matched non-psoriasis subjects (n = 64), from a referral medical centre. The correlation between altered microbiota and disease activity, arthritis and systemic anti-psoriatic drugs was also investigated. We observed a distinct faecal microbial community structure in psoriasis patients, with an increased abundance of phylum Firmicutes and decreased abundance of phylum Bacteroidetes, across different subgroup of subjects. Ruminococcus and Megasphaera, of the phylum Firmicutes, were the top-two genera of discriminant abundance in psoriasis. A number of functional genes and metabolic pathways involving bacterial chemotaxis and carbohydrate transport were predicted over-represented, whereas genes related to cobalamin and iron transport were predicted under-represented in faecal microbiota of psoriasis patients. Conclusions The distinct faecal microbial composition in psoriasis might be associated with altered transport of carbohydrate, cobalamin and iron, as well as chemotaxis.