An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities.
An Exploration of Traditional Chinese Medicinal Plants with Anti-Inflammatory Activities.
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中药植物抗炎活性探索
DOI：
10.1155/2017/1231820
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
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作者：
Fan C;Jin HZ;Wu L;Zhang Y;Ye RD;Zhang W;Zhang Y
为了在中国不断发现更多的抗炎药用植物，我们通过一组体外和体内试验评估了来自 84 种传统药用植物不同部位的 101 种提取物的抗炎活性。在稳定转染的 Hela 细胞中通过荧光素酶测定测定核因子-κ B (NF-κB) 抑制作用。使用MTT测定评估细胞毒性活性。分别通过 Griess 反应和实时 PCR 分析评估对 LPS 诱导的一氧化氮产生和促炎介质的抑制作用。通过二甲苯诱导的小鼠耳水肿模型检查体内抗炎活性。总共 22 种提取物显示出良好的 NF-κB 抑制作用，而其中 9 种提取物不影响细胞活力。 9 种命中提取物在至少一种随后进行的体外药理学测试系统中具有活性。选择小黄花（根）提取物进行体内研究，因为它在所有体外测定中都表现出显着的抑制作用。结果表明，黄花菜（根）提取物能够有效缓解二甲苯诱导的小鼠耳水肿模型。总的来说，我们的研究为中国传统使用的药用植物的潜在抗炎作用提供了证据。进一步的植物化学和药理学研究仍有待澄清。
In a continuing effort to discover more anti-inflammatory medicinal plants in China, the anti-inflammatory activities of 101 extracts from different parts of 84 traditional medicinal plants were evaluated by a panel of in vitro and in vivo assays. Nuclear factor-kappa B (NF-κB) inhibitory effects were determined by luciferase assay in stably transfected Hela cells. Cytotoxic activities were assessed using the MTT assay. Inhibitory effects on LPS-induced nitric oxide production and proinflammatory mediators were assessed by Griess reaction and Real-Time PCR analysis, respectively. In vivo anti-inflammatory activities were examined by xylene-induced mice ear edema model. In total, 22 extracts showed promising NF-κB inhibitory effects whereas 9 of them did not affect the cell viability. The 9 hit extracts were active in at least one of the subsequently performed in vitro pharmacological test systems. The extract from Hemerocallis minor (root) was selected to perform the in vivo study because it demonstrated significant suppressive effects in all the in vitro assays. Results showed that the extract of Hemerocallis minor (Root) was able to alleviate ear edema effectively in xylene-induced mice ear edema mode. Collectively, our study provides evidence for the potential anti-inflammatory effects of the medicinal plants traditionally used in China. Further phytochemical and pharmacological studies remain to be clarified.