An atypical soybean leucine-rich repeat receptor-like kinase, GmLRK1, may be involved in the regulation of cell elongation
An atypical soybean leucine-rich repeat receptor-like kinase, GmLRK1, may be involved in the regulation of cell elongation
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DOI：
10.1007/s00425-008-0873-3
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发表时间：
2009-03-01
期刊：
影响因子：
4.3
通讯作者：
Cheon, Choong-Ill
中科院分区：
文献类型：
--
作者：
Kim, Sunghan;Kim, Su-Jin;Cheon, Choong-Ill
在大豆种子发育的特定阶段高表达的基因之一GmLRK1编码一个富含亮氨酸重复序列的受体样激酶(LRR-RLK)，该基因被鉴定和鉴定。对其激动域的分析表明，GmLRK1可能是一种催化失活的非典型受体激酶。自磷酸化实验证实GmLRK1在体外不能自磷酸化。然而，GmRLK1在与植物蛋白提取物孵育后可以被诱导磷酸化，这表明一些植物蛋白可能在体内与GmLRK1相互作用并磷酸化该蛋白。对GmLRK1及其拟南芥同源基因At2g36570的表达谱分析表明，它们可能参与了更基本的代谢和/或发育途径的调节，而不是种子发育等专门的发育程序。我们的结果进一步表明，GmLRK1和AT2G36570可能在导致细胞伸长和扩张的某些细胞过程中发挥调节作用。
A leucine-rich repeat receptor-like kinase (LRR-RLK) encoded by one of the genes highly expressed in a specific stage of soybean seed development, referred to as GmLRK1, was identified and characterized. Examination of its kinase domain indicated that GmLRK1 may be a catalytically inactive atypical receptor kinase. An autophosphorylation assay confirmed that GmLRK1 is incapable of autophosphorylation in vitro. However, the phosphorylation of GmRLK1 could be induced after incubation with plant protein extracts, suggesting that some plant proteins may interact with GmLRK1 and phosphorylate the protein in vivo. Analyses of the expression profiles of GmLRK1 and its Arabidopsis ortholog At2g36570 revealed that they may be involved in regulation of more fundamental metabolic and/or developmental pathways, rather than a specialized developmental program such as seed development. Our results further indicate that the GmLRK1 and At2g36570 may play a role in the regulation of certain cellular processes that lead to cell elongation and expansion.