Effects of rigid lens extended wear on lactate dehydrogenase activity and isozymes in rabbit tears.
Effects of rigid lens extended wear on lactate dehydrogenase activity and isozymes in rabbit tears.
复制标题
硬性镜片长期佩戴对兔泪液中乳酸脱氢酶活性和同工酶的影响。
DOI：
10.1097/00003226-199409000-00010
复制
发表时间：
1994
期刊：
影响因子：
2.8
通讯作者：
Cavanagh,HD
中科院分区：
文献类型：
--
作者：
Ichijima,H;Cavanagh,HD
通过无创性测定家兔泪液乳酸脱氢酶（LDH）活性和同工酶谱，评价了连续配戴四种硬性透气性角膜接触镜的效果和恢复情况。所用镜片的透氧率（Dk/L）为27、44、84和97 × 10-9（cm/s）（ml O2/ml mmHg）;标准化透镜厚度（0.15 mm）和直径（14.0 mm）。连续配戴镜片90天;在停止配戴透镜后30天评估恢复情况。LDH活性测定采用紫外速率法，同工酶亚型测定采用琼脂糖凝胶电泳法。使用光学显微镜和扫描电子显微镜（LM、SEM）测定总蛋白作为透镜效应的额外测量。LDH水平与透镜Dk/L值呈负相关;低Dk/L值增加了厌氧（LDH 4，5）/需氧（LDH 1，2，3）亚型，比值表明体内代谢变化。SEM观察结果与这些结果一致。对照组和RGP试戴组之间泪液的总细胞含量或总泪液蛋白水平无显著差异。泪液LDH活性和同工酶比率的测量似乎提供了一个敏感的，非侵入性的评估RGP镜片诱导的缺氧随着时间的推移对角膜表面的影响。Dk/L ≥ 84时，在长期配戴过程中维持角膜生理功能最佳。从慢性晶状体诱导的缺氧中恢复的特征在于恢复到正常的泪液LDH水平和同工酶亚型。
Effects of and recovery from continuous wear of four rigid gas permeable (RGP) contact lenses was assessed by noninvasive measurement of lactate dehydrogenase (LDH) activity and isozyme pattern in rabbit tears. Oxygen transmissibility (Dk/L) of lenses used was 27, 44, 84, and 97 x 10-9 (cm/s)(ml O 2/ml mm Hg); lens thickness (0.15 mm) and diameter (14.0 mm) were standardized. Lenses were worn continuously for 90 days; recovery was assessed 30 days after cessation of lens wear. LDH activity was measured by UV rate assay; isozyme subtypes were determined by agarose gel electrophoresis. Light and scanning electron microscopy (LM, SEM) were used with the determination of total protein as additional measures of lens effects. LDH levels were inversely correlated with lens Dk/L values; low Dk/L values increased the anaerobic (LDH 4, 5)/aerobic (LDH 1, 2, 3) subtype,, ratio indicating in vivo metabolic shift. SEM observations were consistent with these results. There was no significant difference in the total cell content of tears or total tear protein levels between control and RGP test-wear groups. Measurement of tear LDH activity and isozyme ratios appears to provide a sensitive, noninvasive assessment of the effects of RGP lens-induced hypoxia over time on the corneal surface. A level of Dk/L of≥ 84appears best for maintaining corneal physiology during extended wear. Recovery from chronic lens-induced hypoxia is characterized by a return to normal tear LDH levels and isozyme subtypes.