DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to methylmercury
DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to methylmercury
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DOI：
10.2131/jts.36.843
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发表时间：
2011-12-01
影响因子：
2
通讯作者：
Kumagai, Yoshito
中科院分区：
文献类型：
--
作者：
Toyama, Takashi;Yoshida, Eiko;Kumagai, Yoshito
为了探讨适应性反应的环境亲电甲基汞（甲基汞），我们进行了DNA微阵列分析的人神经母细胞瘤SH-SY 5 Y细胞暴露于亚细胞毒性剂量的甲基汞（1 μ M）为6 hr. The表达的15个基因增加10倍或更多的响应甲基汞。这些基因中有四个与解毒和排泄甲基汞到细胞外空间，并通过亲电反应元件由转录因子Nrf 2调节。有趣的是，Cullin 3，Nrf 2的负调节因子，被确定为在甲基汞暴露过程中下调的基因。
To investigate the adaptive response to the environmental electrophile methylmercury (MeHg), we performed DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to a sub-cytotoxic dose of MeHg (1 mu M) for 6 hr. The expression of 15 genes increased 10-fold or more in response to MeHg. Four of these genes are associated with detoxification and excretion of MeHg into the extracellular space, and are regulated by transcription factor Nrf2 through the electrophile response element. Interestingly, Cullin3, a negative regulator of Nrf2, was identified as a down-regulated gene during MeHg exposure.