CONSTRUCTION OF AN SFII MACRORESTRICTION MAP OF THE CANDIDA-ALBICANS GENOME
CONSTRUCTION OF AN SFII MACRORESTRICTION MAP OF THE CANDIDA-ALBICANS GENOME
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DOI：
10.1128/jb.175.20.6637-6651.1993
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发表时间：
1993-10-01
影响因子：
3.2
通讯作者：
MAGEE, PT
中科院分区：
文献类型：
--
作者：
CHU, WS;MAGEE, BB;MAGEE, PT
条件致病真菌白色念珠菌是二倍体，通常是孤立的，没有明显的性周期。因此，基因分析非常困难。在过去的几年里，分子遗传学已经产生了重要的信息，但它也受到缺乏良好的遗传图谱的阻碍。利用已鉴定的菌株1006和经历白色-不透明表型转变的菌株WO-1，我们开发了C.白色念珠菌的SfiI酶。在C.白色念珠菌基因组。通过脉冲场电泳分离限制性片段，并通过用已知的染色体特异性探针杂交完整和部分引物以及消化分离的染色体将其分配到染色体上。端粒片段通过与端粒特异性探针（C. Sadhu，M. J. McEachern，E. P. Rustchenko-Bulgac，J. Schmid，D. R. Soll和J. B. Hicks，J. Bacteriol. 173：842-850，1991）。WO-1与1006的不同之处在于它经历了三次相互染色体易位。易位产物的分析表明，每个易位发生在SfiI位点或其附近;因此，来自两个菌株的SfiI片段相似或相同。在SfiI位点或其附近发生易位的趋势可能与重复序列RPS 1有关，RPS 1含有4个这样的位点，可以为异位配对和交换提供同源性。这两种菌株的基因组大小约为16至17兆碱基，与先前的测定结果非常一致。
The opportunistic fungal pathogen, Candida albicans, is diploid is usually isolated and has no apparent sexual cycle. Genetic analysis has therefore been very difficult. Molecular genetics has yielded important information in the past few years, but it too is hampered by the lack of a good genetic map. Using the well-characterized strain 1006 and strain WO-1, which undergoes the white-opaque phenotypic transition, we have developed a genomic restriction map of C. albicans with the enzyme SfiI. There are approximately 34 SfiI restriction sites in the C. albicans genome. Restriction fragments were separated by pulsed-field electrophoresis and were assigned to chromosomes by hybridization of complete and partial digests with known chromosome-specific probes as well as by digestion of isolated chromosomes. Telomeric fragments were identified by hybridization with a telomere-specific probe (C. Sadhu, M. J. McEachern, E. P. Rustchenko-Bulgac, J. Schmid, D. R. Soll, and J. B. Hicks, J. Bacteriol. 173:842-850, 1991). WO-1 differs from 1006 in that it has undergone three reciprocal chromosomal translocations. Analysis of the translocation products indicates that each translocation has occurred at or near an SfiI site; thus, the SfiI fragments from the two strains are similar or identical. The tendency for translocation to occur at or near SfiI sites may be related to the repeated sequence RPS 1, which contains four such sites and could provide homology for ectopic pairing and crossing over. The genome size of both strains is about 16 to 17 megabases, in good agreement with previous determinations.