Combined assay of CYFRA21-1, telomerase and vascular endothelial growth factor in the detection of bladder transitional cell carcinoma
Combined assay of CYFRA21-1, telomerase and vascular endothelial growth factor in the detection of bladder transitional cell carcinoma
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DOI：
10.1111/j.1442-2042.2007.01670.x
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发表时间：
2007-02-01
影响因子：
2.6
通讯作者：
Xu, Zhishun
中科院分区：
文献类型：
--
作者：
Bian, Wei;Xu, Zhishun
背景：CYFRA21-1、端粒酶和血管内皮生长因子（VEGF）被认为是检测膀胱移行细胞癌的有用肿瘤标志物。然而，每个单一标记的敏感性似乎都不令人满意。在目前的研究中，我们评估了这三种标志物联合检测人类膀胱移行细胞癌的敏感性。方法：收集100例膀胱移行细胞癌患者的空尿标本，并对其进行各项检测。采用酶联免疫吸附法检测尿CYFRA21-1和VEGF，端粒重复扩增法检测端粒酶。将联合检测的灵敏度分别与各单项标志物和尿细胞学检测的灵敏度进行比较。结果：100例患者中，尿CYFRA21-1敏感性为74%，端粒酶敏感性为71%，VEGF敏感性为69%，细胞学敏感性为38%。CYFRA21-1、端粒酶和VEGF的敏感性均显著高于细胞学（P = 0.000）。本研究联合检测的灵敏度为94%，显著高于细胞学、尿端粒酶、CYFRA21-1或VEGF检测（P = 0.000）。在50例血尿但无膀胱癌的患者中，CYFRA21-1检测的总体特异性为78%，端粒酶检测为84%，VEGF检测为88%，细胞学检测为92%。结论：联合检测显示不同肿瘤行为特征的标志物对膀胱移行细胞癌的检测有重要意义。
Background: CYFRA21-1, telomerase and vascular endothelial growth factor (VEGF) are regarded as useful tumor markers in the detection of bladder transitional cell carcinoma. However, the sensitivity of each single marker seemed to be unsatisfactory. In the current study, we assessed the sensitivity of the combined assay of these three markers in the detection of human bladder transitional cell carcinoma.Methods: Voided urine samples of 100 patients with bladder transitional cell carcinoma were obtained and each sample was aliquoted for the various assay. Urinary CYFRA21-1 and VEGF were detected by enzyme-linked immunosorbent assay and telomerase detected by the telomeric repeat amplification protocol. The sensitivity of combined assay was compared to that of each single marker and urinary cytology, respectively.Results: In the 100 patients, the sensitivity was 74% for urinary CYFRA21-1, 71% for telomerase, 69% for VEGF, and 38% for cytology. CYFRA21-1, telomerase and VEGF proved significantly more sensitive than cytology, respectively (P = 0.000). The sensitivity of the combined assay in the current study was 94% and it was significantly higher than that of cytology, urinary telomerase, CYFRA21-1 or VEGF (P = 0.000). In 50 patients with hematuria but without bladder cancer, the overall specificity of the assays was 78% for CYFRA21-1, 84% for telomerase, 88% for VEGF, and 92% for cytology.Conclusions: Combined assay of the markers which show different characteristics of the tumor behaviors seemed to be of interest in the detection of bladder transitional cell carcinoma.